Literature summary for 3.5.1.B4 extracted from

Shi, D.; Yu, X.; Roth, L.; Tuchman, M.; Allewell, N.M.
Structure of a novel N-acetyl-L-citrulline deacetylase from Xanthomonas campestris (2007), Biophys. Chem., 126, 86-93.

Search for more literature for 3.5.1.B4

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)	Xanthomonas campestris

Crystallization (Commentary)

Crystallization (Comment)	Organism
His-tag free protein, hanging drop vapor diffusion method	Xanthomonas campestris

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	bound to active site, coordinated by His72, Asp103, Glu155, and one water molecule	Xanthomonas campestris

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N2-acetyl-L-citrulline + H2O	Xanthomonas campestris	involved in an alternative pathway for arginine synthesis	L-citrulline + acetate	-	?

Organism

Organism	UniProt	Comment	Textmining
Xanthomonas campestris	-	strain ATCC 33913D	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein using His-tag	Xanthomonas campestris

Reaction

Reaction	Comment	Organism	Reaction ID
N-acetyl-L-citrulline + H2O = L-citrulline + acetate	Glu130 functions as a general acidbase catalyst, activating a water molecule, while the Co2+ ion interacts with the acetyl group to polarize the amide bond	Xanthomonas campestris	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N2-acetyl-L-citrulline + H2O	-	Xanthomonas campestris	L-citrulline + acetate	-	?
N2-acetyl-L-citrulline + H2O	involved in an alternative pathway for arginine synthesis	Xanthomonas campestris	L-citrulline + acetate	-	?

Subunits

Subunits	Comment	Organism
dimer	crystal structure analysis	Xanthomonas campestris

Synonyms

Synonyms	Comment	Organism
aCDase	-	Xanthomonas campestris
argE' gene product	-	Xanthomonas campestris

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Release 2023.1 (January 2023)