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Literature summary for 3.5.1.98 extracted from

  • Kutil, Z.; Skultetyova, L.; Rauh, D.; Meleshin, M.; Snajdr, I.; Novakova, Z.; Mikesova, J.; Pavlicek, J.; Hadzima, M.; Baranova, P.; Havlinova, B.; Majer, P.; Schutkowski, M.; Barinka, C.
    The unraveling of substrate specificity of histone deacetylase 6 domains using acetylome peptide microarrays and peptide libraries (2019), FASEB J., 33, 4035-4045 .
    View publication on PubMed
Search for more literature for 3.5.1.98

Protein Variants

Protein Variants Comment Organism
H216A site-directed mutagenesis, catalytically almost inactive DD1 domain mutant Homo sapiens
H216A/H611A site-directed mutagenesis, catalytically inactive DD1/DD2 domains mutant Homo sapiens
H611A site-directed mutagenesis, catalytically almost inactive DD2 domain mutant Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics Homo sapiens
0.0056
-
 TYSRGGK(Ac)FFRRFP pH 7.4, 37°C, recombinant enzyme Homo sapiens
0.0104
-
 YSRGGK(Ac)FFRRF pH 7.4, 37°C, recombinant enzyme Homo sapiens
0.0122
-
 SRGGK(Ac)FFRR pH 7.4, 37°C, recombinant enzyme Homo sapiens
0.0128
-
 RGGK(Ac)FFR pH 7.4, 37°C, recombinant enzyme Homo sapiens
0.0195
-
 RYSSRRK(Ac)ARPYML pH 7.4, 37°C, recombinant enzyme Homo sapiens
0.021
-
 SQSEEEK(Ac)FFRRFP pH 7.4, 37°C, recombinant enzyme Homo sapiens
0.0351
-
 GGK(Ac)FF pH 7.4, 37°C, recombinant enzyme Homo sapiens
0.0517
-
 GK(Ac)F pH 7.4, 37°C, recombinant enzyme Homo sapiens
0.0687
-
 TYSRGGK(Ac)KSEESE pH 7.4, 37°C, recombinant enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ zinc-dependent Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UBN7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AAKAVGK(Ac)VIPELN + H2O
-
 Homo sapiens AAKAVGKVIPELN + acetate
-
 ?
AFKFFQK(Ac)DRKMAL + H2O
-
 Homo sapiens AFKFFQKDRKMAL + acetate
-
 ?
ASKRAFK(Ac)QSPPAV + H2O
-
 Homo sapiens ASKRAFKQSPPAV + acetate
-
 ?
DPGNEVK(Ac)LKLYAL + H2O
-
 Homo sapiens DPGNEVKLKLYAL + acetate
-
 ?
EANPGEK(Ac)RKMTDS + H2O
-
 Homo sapiens EANPGEKRKMTDS + acetate
-
 ?
GATYQDK(Ac)RYTNKY + H2O
-
 Homo sapiens GATYQDKRYTNKY + acetate
-
 ?
GFGNGMK(Ac)QMRRTW + H2O
-
 Homo sapiens GFGNGMKQMRRTW + acetate
-
 ?
GGK(Ac)FF + H2O
-
 Homo sapiens GGKFF + acetate
-
 ?
GGMRGMK(Ac)GLVYET + H2O
-
 Homo sapiens GGMRGMKGLVYET + acetate
-
 ?
GK(Ac)F + H2O
-
 Homo sapiens GKF + acetate
-
 ?
HGGTILK(Ac)MIEEAG + H2O
-
 Homo sapiens HGGTILKMIEEAG + acetate
-
 ?
LYDRGTK(Ac)FGLFTP + H2O
-
 Homo sapiens LYDRGTKFGLFTP + acetate
-
 ?
additional information histone deacetylase 6 (HDAC6) is a multidomain cytosolic hydrolase acting mostly on non-histone protein substrates. The substrate specificity of HDAC6 is influenced by the presence of two catalytically active deacetylase domains, DD1 and DD2. Substrate specificity analysis of HDAC6 domains using acetylome peptide microarrays and peptide libraries using a peptide microarray displaying over 6800 peptides derived from known human acetylation sites, profiling of HDAC6 deacylation specificity, overview. No activity with SQSEEEK(Ac)KSEESE and SQSEVKK(Ac)PSEESE. The acetylation sites are collected from previously published data, synthesized as 13 mer derivatives with an N-terminal reactivity tag, and immobilized onto modified glass slides via a covalent bond. Residues N- and C-terminal to the central Lys(Ac) are required for efficient substrate recognition by HDAC6. The DD1 domain is unable to remove the acetyl group from the central lysine residue in the context of 13 mer peptides attached to the glass surface Homo sapiens ?
-
-
NARRKPK(Ac)GSPRVP + H2O
-
 Homo sapiens NARRKPKGSPRVP + acetate
-
 ?
QSVQPSK(Ac)FNSYGR + H2O
-
 Homo sapiens QSVQPSKFNSYGR + acetate
-
 ?
QTSPNPK(Ac)YRGFFH + H2O
-
 Homo sapiens QTSPNPKYRGFFH + acetate
-
 ?
RGGK(Ac)FFR + H2O
-
 Homo sapiens RGGKFFR + acetate
-
 ?
RIANRTK(Ac)NSLTLQ + H2O
-
 Homo sapiens RIANRTKNSLTLQ + acetate
-
 ?
RINSGGK(Ac)LPNFGF + H2O
-
 Homo sapiens RINSGGKLPNFGF + acetate
-
 ?
RSLKYGK(Ac)VSPSLV + H2O
-
 Homo sapiens RSLKYGKVSPSLV + acetate
-
 ?
RTSGRDK(Ac)YGPPVR + H2O
-
 Homo sapiens RTSGRDKYGPPVR + acetate
-
 ?
RTSLGPK(Ac)SMMKML + H2O
-
 Homo sapiens RTSLGPKSMMKML + acetate
-
 ?
RYSSRRK(Ac)ARPYML + H2O
-
 Homo sapiens RYSSRRKARPYML + acetate
-
 ?
SQSEEEK(Ac)FFRRFP + H2O
-
 Homo sapiens SQSEEEKFFRRFP + acetate
-
 ?
SRGGK(Ac)FFRR + H2O
-
 Homo sapiens SRGGKFFRR + acetate
-
 ?
STPRFGK(Ac)TFDAPP + H2O
-
 Homo sapiens STPRFGKTFDAPP + acetate
-
 ?
TGKYFDK(Ac)ASYRVY + H2O
-
 Homo sapiens TGKYFDKASYRVY + acetate
-
 ?
TLKKTGK(Ac)TVSYLG + H2O
-
 Homo sapiens TLKKTGKTVSYLG + acetate
-
 ?
TYSRGGK(Ac)FFRRFP + H2O
-
 Homo sapiens TYSRGGKFFRRFP + acetate
-
 ?
TYSRGGK(Ac)KSEESE + H2O
-
 Homo sapiens TYSRGGKKSEESE + acetate
-
 ?
VLRNGGK(Ac)NFPAIF + H2O
-
 Homo sapiens VLRNGGKNFPAIF + acetate
-
 ?
VRGFGGK(Ac)FGVQMD + H2O
-
 Homo sapiens VRGFGGKFGVQMD + acetate
-
 ?
WEALGGK(Ac)AAYRTS + H2O
-
 Homo sapiens WEALGGKAAYRTS + acetate
-
 ?
YSRGGK(Ac)FFRRF + H2O
-
 Homo sapiens YSRGGKFFRRF + acetate
-
 ?

Synonyms

Synonyms Comment Organism
HDAC6
-
 Homo sapiens
histone deacetylase 6
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 SQSEEEK(Ac)FFRRFP pH 7.4, 37°C, recombinant enzyme Homo sapiens
0.12
-
 TYSRGGK(Ac)KSEESE pH 7.4, 37°C, recombinant enzyme Homo sapiens
0.71
-
 TYSRGGK(Ac)FFRRFP pH 7.4, 37°C, recombinant enzyme Homo sapiens
0.92
-
 RYSSRRK(Ac)ARPYML pH 7.4, 37°C, recombinant enzyme Homo sapiens
1.09
-
 GGK(Ac)FF pH 7.4, 37°C, recombinant enzyme Homo sapiens
1.53
-
 RGGK(Ac)FFR pH 7.4, 37°C, recombinant enzyme Homo sapiens
1.66
-
 YSRGGK(Ac)FFRRF pH 7.4, 37°C, recombinant enzyme Homo sapiens
1.88
-
 GK(Ac)F pH 7.4, 37°C, recombinant enzyme Homo sapiens
2.28
-
 SRGGK(Ac)FFRR pH 7.4, 37°C, recombinant enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.95
-
 SQSEEEK(Ac)FFRRFP pH 7.4, 37°C, recombinant enzyme Homo sapiens
1.75
-
 TYSRGGK(Ac)KSEESE pH 7.4, 37°C, recombinant enzyme Homo sapiens
31.1
-
 GGK(Ac)FF pH 7.4, 37°C, recombinant enzyme Homo sapiens
36.36
-
 GK(Ac)F pH 7.4, 37°C, recombinant enzyme Homo sapiens
47.18
-
 RYSSRRK(Ac)ARPYML pH 7.4, 37°C, recombinant enzyme Homo sapiens
119.5
-
 RGGK(Ac)FFR pH 7.4, 37°C, recombinant enzyme Homo sapiens
126.78
-
 TYSRGGK(Ac)FFRRFP pH 7.4, 37°C, recombinant enzyme Homo sapiens
159.6
-
 YSRGGK(Ac)FFRRF pH 7.4, 37°C, recombinant enzyme Homo sapiens
186.9
-
 SRGGK(Ac)FFRR pH 7.4, 37°C, recombinant enzyme Homo sapiens