Literature summary for 3.5.1.98 extracted from

Stefan, A.; Calonghi, N.; Schipani, F.; Dal Piaz, F.; Sartor, G.; Hochkoeppler, A.
Purification of active recombinant human histone deacetylase 1 (HDAC1) overexpressed in Escherichia coli (2018), Biotechnol. Lett., 40, 1355-1363 .

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Cloned(Commentary)

Cloned (Comment)	Organism
a synthetic gene coding for HDAC1 is optimised for Escherichia coli codon usage, cloning into pBADHisB, generating vector pBAD-rHDAC1, and recombinant overexpression in Escherichia coli strain TOP10, method evaluation. The overexpression of HDAC1 is induced for 15 h at 30°C with 1.3 mM arabinose, when bacterial populations reach an OD600 equal to 0.8-0.9	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
110000	-	dimeric recombinant enzyme, gel filtration	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q13547	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant HDAC1 from Escherichia coli strain TOP10 by anion exchange chromatography, ultrafiltration, heparin affinity chromatography, poly-L-lysine affinity chromatography, and gel filtration. The effects of chaotropic species, weak kosmotropes, amino acids, reducing or denaturing agents, detergents, alcohols, and saturated fatty acids are tested to dissociate the protein aggregates, overview	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	substrate are acetate-labeled histones from HT-29 cells	Homo sapiens	?	-	-

Subunits

Subunits	Comment	Organism
dimer	2 * 55000, recombinant enzyme, SDS-PAGE	Homo sapiens
More	similar histones substrate conversion is observed in the presence of octameric and monomeric enzyme	Homo sapiens

Synonyms

Synonyms	Comment	Organism
HDAC1	-	Homo sapiens
histone deacetylase 1	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
evolution	human histone deacetylases belong to all three known classes, class I: HDAC1, HDAC2, HDAC3, HDAC8, class II: HDAC4, HDAC5, HDAC6, HDAC7, HDAC9, HDAC10, and class III: HDAC11	Homo sapiens
additional information	similar histones substrate conversion is observed in the presence of octameric and monomeric enzyme	Homo sapiens
physiological function	histone deacetylases (HDACs) are specifically responsible for the deacetylation of lysine residues at the N-terminal regions of the core histones (H2A, H2B, H3 and H4). In addition, HDACs may deacetylate other non-histone proteins and thus are involved in several cellular processes (e.g. differentiation, apoptosis, cancer development)	Homo sapiens

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Release 2023.1 (January 2023)