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Literature summary for 3.5.1.98 extracted from

  • Karthikeyan, G.; Paul-Satyaseela, M.; Dhatchana Moorthy, N.; Gopalaswamy, R.; Narayanan, S.
    Functional characterization of Candida albicans Hos2 histone deacetylase (2013), F1000Res., 2, 238.
    View publication on PubMed
Search for more literature for 3.5.1.98

Cloned(Commentary)

Cloned (Comment) Organism
gene HOS2, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-atgegd enzyme in Spodoptera frugiperda Sf9 cells Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
MGCD 290 an enzyme Hos2 inhibitor, for use in combination with azoles, such as fluconazole, for fungal infections Candida albicans
MS-275 56.3% inhibition at 0.01 mM, 46.3% inhibition at 0.001 mM Candida albicans
suberoylanilide hydroxamic acid
-
 Candida albicans
trichostatin A
-
 Candida albicans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-lysine-histone + H2O Candida albicans
-
 acetate + histone
-
 ?
N-acetyl-lysine-histone + H2O Candida albicans ATCC 90028
-
 acetate + histone
-
 ?

Organism

Organism UniProt Comment Textmining
Candida albicans
-
 gene HOS2
-
Candida albicans ATCC 90028
-
 gene HOS2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-atgegd enzyme from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography and dialysis Candida albicans

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Candida albicans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-acetyl-Lys-7-amido-4-methylcoumarin + H2O
-
 Candida albicans Boc-acetyl-Lys + 7-amino-4-methylcoumarin
-
 ?
Boc-acetyl-Lys-7-amido-4-methylcoumarin + H2O
-
 Candida albicans ATCC 90028 Boc-acetyl-Lys + 7-amino-4-methylcoumarin
-
 ?
additional information purified fungal Hos2 protein consistently deacetylates tubulins, rather than histones from trichostatin A-treated cells. Hos2 does not show any sirtuin activity, the enzyme deacetylates human nuclear histones from HeLa cells and human acetylated tubulin from Jurkat cells, overview Candida albicans ?
-
 ?
additional information purified fungal Hos2 protein consistently deacetylates tubulins, rather than histones from trichostatin A-treated cells. Hos2 does not show any sirtuin activity, the enzyme deacetylates human nuclear histones from HeLa cells and human acetylated tubulin from Jurkat cells, overview Candida albicans ATCC 90028 ?
-
 ?
N-acetyl-lysine-histone + H2O
-
 Candida albicans acetate + histone
-
 ?
N-acetyl-lysine-histone + H2O
-
 Candida albicans ATCC 90028 acetate + histone
-
 ?

Synonyms

Synonyms Comment Organism
class I HDAC
-
 Candida albicans
HDAC
-
 Candida albicans
Hos2
-
 Candida albicans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Candida albicans

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000028
-
 pH 8.0, 37°C, recombinant enzyme Candida albicans trichostatin A
0.000065
-
 pH 8.0, 37°C, recombinant enzyme Candida albicans suberoylanilide hydroxamic acid

General Information

General Information Comment Organism
malfunction deletion of Hos2, the catalytic subunit of the Set3 complex, produces a phenotype resembling inhibition of the Set3C by trichostatin-A Candida albicans
additional information enzyme Hos2 is not inhibited by class I inhibitors such as MS-275 Candida albicans
physiological function the Hos2/Set3 histone deacetylase complex (Set3C) plays a key role in the conversion of white phase to virulent opaque phase in Candida albicans. Azole resistance can be reversed by the co-administration of a histone deacetylase inhibitor, suggesting that resistance is mediated by epigenetic mechanisms possibly involving the fungal deacetylase Hos2 Candida albicans