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Literature summary for 3.5.1.98 extracted from

  • Osoata, G.O.; Yamamura, S.; Ito, M.; Vuppusetty, C.; Adcock, I.M.; Barnes, P.J.; Ito, K.
    Nitration of distinct tyrosine residues causes inactivation of histone deacetylase 2 (2009), Biochem. Biophys. Res. Commun., 384, 366-371.
    View publication on PubMed
Search for more literature for 3.5.1.98

Application

Application Comment Organism
medicine HDAC2 is nitrated under nitrative/oxidative stress and in the peripheral lung tissues of smokers and patients with chronic obstructive pulmonary disease Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y146A mutant, constructed for identification of tyrosine residues responsible for HDAC2 degradation Homo sapiens
Y146A in presence of peroxynitrite, activity is reduced by 32%. In presence of 3-morpholinosydonomine, activity is reduced similar to wild-type Homo sapiens
Y153A mutant, constructed for identification of tyrosine residues responsible for HDAC2 degradation Homo sapiens
Y153A in presence of 3-morpholinosydonomine, activity is reduced similar to wild-type. In presence of peroxynitrite, activity is abolished Homo sapiens
Y253A mutant, constructed for identification of tyrosine residues responsible for HDAC2 degradation Homo sapiens
Y253A Y253 is at least partly responsible for the proteasomal degradation of HDAC2 under nitrative stress. Activity is not reduced in presence of 3-morpholinosydonomine Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information nitration of distinct tyrosine residues; nitrative/oxidative stress reduce HDAC2 expression via nitration of distinct tyrosine residues. Peroxynitrite, hydrogen peroxide and cigarette smoke-conditioned medium reduce HDAC2 expression in A549 epithelial cells in vitro. This reduction is due to increased proteasomal degradation following ubiquitination rather than reduction of mRNA expression or stability Homo sapiens
peroxynitrite activity of wild-type enzyme is reduced to 38% in presence of peroxynitrite. Activities of mutants Y153A and Y253A are 233 completely abolished in the presence of peroxynitrite. Mutant Y146A shows 32% reduction in activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 determined by SDS-PAGE and Western Blot analysis Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens Q92769
-
-

Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell
-
 Homo sapiens
-
lung
-
 Homo sapiens
-
U2-OS cell
-
 Homo sapiens
-

Synonyms

Synonyms Comment Organism
HDAC2
-
 Homo sapiens
histone deacetylase 2
-
 Homo sapiens

General Information

General Information Comment Organism
metabolism nitrative/oxidative stress reduce HDAC2 expression via nitration of distinct tyrosine residues. Peroxynitrite, hydrogen peroxide and cigarette smoke-conditioned medium reduce HDAC2 expression in A549 epithelial cells in vitro. This reduction is due to increased proteasomal degradation following ubiquitination rather than reduction of mRNA expression or stability Homo sapiens