Literature summary for 3.5.1.98 extracted from

Pflum, M.K.H.; Tong, J.K.; Lane, W.S.; Schreiber, S.L.
Histone deacetylase 1 phosphorylation promotes enzymatic activity and complex formation (2001), J. Biol. Chem., 276, 47733-47741.

Search for more literature for 3.5.1.98

Cloned(Commentary)

Cloned (Comment)	Organism
expression of FLAG-tagged enzyme in T-Ag Jurkat cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	deletion of casein kinase 2 consensus sequence resulting in loss of phosphorylation reduces the enzyme activity and complex formation. Deletion of the highly charged C-terminal region decreases catalytic activity to 22% of wild-type and protein associations	Homo sapiens
S421A	mutation of phosphorylation site, reduces the enzyme activity to 33% and disturbs complex formation. Mutant activates transcription of luciferase reporter gene under conditions where the wild-type does not activate	Homo sapiens
S421A/S423A	mutation of phosphorylation sites, reduces the enzyme activity to 22% and disturbs complex formation. Mutant activates transcription of luciferase reporter gene under conditions where the wild-type does not activate	Homo sapiens
S421D	mutation of phosphorylation site, 82.5% of wild-type activity	Homo sapiens
S421D/S423D	mutation of phosphorylation site, 34% of wild-type activity	Homo sapiens
S421E	mutation of phosphorylation site, 81.8% of wild-type activity	Homo sapiens
S421E/S423E	mutation of phosphorylation site, 38.5% of wild-type activity	Homo sapiens
S423A	mutation of phosphorylation site, reduces the enzyme activity to 25% and disturbs complex formation. Mutant activates transcription of luciferase reporter gene under conditions where the wild-type does not activate	Homo sapiens
S423D	mutation of phosphorylation site, 40% of wild-type activity	Homo sapiens
S423E	mutation of phosphorylation site, 48% of wild-type activity	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
trapoxin	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	isoform HDAC1	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	isoform HDAC1 is phosphorylated at S423 and S421. Loss of phosphorylation reduces the enzyme acivity and complex formation	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
via affinity chromatography using irreversible inhibitor trapoxin	Homo sapiens

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Release 2023.1 (January 2023)