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Literature summary for 3.5.1.98 extracted from

  • Song, Y.M.; Kim, Y.S.; Kim, D.; Lee, D.S.; Kwon, H.J.
    Cloning, expression, and biochemical characterization of a new histone deacetylase-like protein from Thermus caldophilus GK24 (2007), Biochem. Biophys. Res. Commun., 361, 55-61.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli as glutathione S-transferase fusion protein Thermus caldophilus

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 30 min, complete loss of activity. Zn2+, Mg2+, Mn2+ may restore activity Thermus caldophilus
trichostatin A
-
Thermus caldophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km value according to commercial HDAC Fluorescent Activity Assay Kit is 0.143 mM Thermus caldophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Zn2+, Mg2+, or Mn2+ is required. Zn2+ is the most efficient Thermus caldophilus
Mn2+ Zn2+, Mg2+, or Mn2+ is required. Zn2+ is the most efficient Thermus caldophilus
Zn2+ Zn2+, Mg2+, or Mn2+ is required. Zn2+ is the most efficient Thermus caldophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42188
-
x * 42188, calculated Thermus caldophilus

Organism

Organism UniProt Comment Textmining
Thermus caldophilus
-
-
-
Thermus caldophilus GK24
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Thermus caldophilus

Subunits

Subunits Comment Organism
? x * 42188, calculated Thermus caldophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Thermus caldophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Thermus caldophilus