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Literature summary for 3.5.1.97 extracted from

  • Li, Y.; Zhao, J.; Quan, C.; Jin, L.; Xu, Y.; Chen, M.
    Production, characterization, and powder preparation of quorum quenching acylase AiiO for pathogen control (2019), Prep. Biochem. Biotechnol., 49, 151-157.
    View publication on PubMed
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Application

Application Comment Organism
medicine use of acylase powder can decrease the expression levels of virulence-related factors of Pseudomonas aeruginosa Ochrobactrum quorumnocens
synthesis production by expression in Escherichia coli, using auto-induction and temperature regulation. For the soluble AiiO protein in the cells, maximum production of 214.3 mg/l is obtained in the fermenter Ochrobactrum quorumnocens

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged protein in Escherichia coli Ochrobactrum quorumnocens

Organism

Organism UniProt Comment Textmining
Ochrobactrum quorumnocens D8X182
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.2
-
 pH 7, 30°C Ochrobactrum quorumnocens

Storage Stability

Storage Stability Organism
4°C, sucrose as the protective agent of the acylase powder, 89.6 and 71.9% of initial specific activity after storage for 3 and 6 months, respectively Ochrobactrum quorumnocens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-hexanoyl-DL-homoserine lactone + H2O
-
 Ochrobactrum quorumnocens homoserine lactone + hexanoate
-
 ?

Synonyms

Synonyms Comment Organism
AiiO
-
 Ochrobactrum quorumnocens