Application | Comment | Organism |
---|---|---|
biotechnology | the enzyme is useful as a biosensor | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain DH10B | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | virulence of the opportunistic human pathogen is controlled by the N-acyl-homoserine lactone, AHL-dependent quorum-sensing system, the enzyme inactivates AHLs by hydrolysis of the carboxylic side chain with side chain ranging from 11 to 14 C-atoms with broad range specificity, addition of the purified enzyme to cell cultures results in complete inhibition of N-3-oxododecanoyl-(S)-homoserine lactone accumulation and production of the signal molecule 2-heptyl-3-hydroxy-4(1H)-quinolone, as well as reduced production of virulence factors elastase and procyanin | ? | - |
? | |
N-3-oxododecanoyl-(S)-homoserine lactone + H2O | Pseudomonas aeruginosa | - |
(S)-homoserine + 3-oxododecanoic acid | - |
? | |
N-dodecanoyl-(S)-homoserine lactone + H2O | Pseudomonas aeruginosa | - |
(S)-homoserine + dodecanoic acid | - |
? | |
N-tetradecanoyl-(S)-homoserine lactone + H2O | Pseudomonas aeruginosa | - |
(S)-homoserine + tetradecanoic acid | - |
? | |
N-undecanoyl-(S)-homoserine lactone + H2O | Pseudomonas aeruginosa | - |
(S)-homoserine + undecanoic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
gene PA2385 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain DH10B by several chromatographic steps | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | virulence of the opportunistic human pathogen is controlled by the N-acyl-homoserine lactone, AHL-dependent quorum-sensing system, the enzyme inactivates AHLs by hydrolysis of the carboxylic side chain with side chain ranging from 11 to 14 C-atoms with broad range specificity, addition of the purified enzyme to cell cultures results in complete inhibition of N-3-oxododecanoyl-(S)-homoserine lactone accumulation and production of the signal molecule 2-heptyl-3-hydroxy-4(1H)-quinolone, as well as reduced production of virulence factors elastase and procyanin | Pseudomonas aeruginosa | ? | - |
? | |
additional information | N-acyl-homoserine lactone substrate specificity, no activity with N-butanoyl-(S)-homoserine lactone, N-hexanoyl-(S)-homoserine lactone, and N-3-oxohexanoyl-(S)-homoserine lactone, overview | Pseudomonas aeruginosa | ? | - |
? | |
N-3-oxodecanoyl-(S)-homoserine lactone + H2O | - |
Pseudomonas aeruginosa | (S)-homoserine lactone + 3-oxodecanoic acid | - |
? | |
N-3-oxododecanoyl-(S)-homoserine lactone + H2O | - |
Pseudomonas aeruginosa | (S)-homoserine + 3-oxododecanoic acid | - |
? | |
N-dodecanoyl-(S)-homoserine lactone + H2O | - |
Pseudomonas aeruginosa | (S)-homoserine + dodecanoic acid | - |
? | |
N-heptanoyl-(S)-homoserine lactone + H2O | - |
Pseudomonas aeruginosa | (S)-homoserine + heptanoic acid | - |
? | |
N-octanoyl-(S)-homoserine lactone + H2O | - |
Pseudomonas aeruginosa | (S)-homoserine + octanoic acid | - |
? | |
N-tetradecanoyl-(S)-homoserine lactone + H2O | - |
Pseudomonas aeruginosa | (S)-homoserine + tetradecanoic acid | - |
? | |
N-undecanoyl-(S)-homoserine lactone + H2O | - |
Pseudomonas aeruginosa | (S)-homoserine + undecanoic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the N-terminal nucleophile hydrolase superfamily | Pseudomonas aeruginosa |
N-acyl-homoserine lactone acylase | - |
Pseudomonas aeruginosa |
PA2385 protein | - |
Pseudomonas aeruginosa |
quorum-quenching AHL acylase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Pseudomonas aeruginosa |