Cloned (Comment) | Organism |
---|---|
recombinant enzyme expression in Escherichia coli strain BL21(DE3)/pET-CPCAcy | Pseudomonas sp. SE83 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the recombinant enzyme is immobilized, microenvironment with an acidic pH (about pH 6-7) during the catalysis of cephalosporin C with the immobilized enzyme. The immobilization efficiency is increased when the enzyme is immobilized in the presence of inhibitors sodium acetate and sodium bicarbobate. And the immobilized enzyme, in the presence of inhibitors, has a higher thermostability than the control at 50°C | Pseudomonas sp. SE83 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-aminopenicillanic acid | 6-APA, a noncompetitive inhibitor | Pseudomonas sp. SE83 | |
acetate | - |
Pseudomonas sp. SE83 | |
bicarbonate | - |
Pseudomonas sp. SE83 | |
Butyrate | - |
Pseudomonas sp. SE83 | |
ethanolamine | - |
Pseudomonas sp. SE83 | |
formate | - |
Pseudomonas sp. SE83 | |
glucosamine | - |
Pseudomonas sp. SE83 | |
Lactate | - |
Pseudomonas sp. SE83 | |
additional information | effects of the competitive inhibitors on the catalysis and immobilization of cephalosporin C acylase, mechanism of competitive inhibition, overview | Pseudomonas sp. SE83 | |
propionate | - |
Pseudomonas sp. SE83 | |
Pyridine | - |
Pseudomonas sp. SE83 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O | Pseudomonas sp. SE83 | - |
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. SE83 | P15557 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O | - |
Pseudomonas sp. SE83 | 7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AcyI | - |
Pseudomonas sp. SE83 |
acylase ACY 1 proenzyme | UniProt | Pseudomonas sp. SE83 |
CCA | - |
Pseudomonas sp. SE83 |
cephalosporin C acylase | - |
Pseudomonas sp. SE83 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
recombinant immobilized enzyme, assay at | Pseudomonas sp. SE83 |
37 | - |
recombinant free enzyme, assay at | Pseudomonas sp. SE83 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the immobilized enzyme, in the presence of inhibitors, has a higher thermostability than the control. The immobilized enzyme with sodium acetate shows a 31.7% increase in activity and a 25% increase with sodium bicarbonate. The increased stability and activity recovery in the process of immobilization might be attributed to the increase in the enzyme rigidity via binding of inhibitors with CCA at the active sites | Pseudomonas sp. SE83 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant free enzyme, assay at | Pseudomonas sp. SE83 |
8.5 | - |
recombinant immobilized enzyme, assay at | Pseudomonas sp. SE83 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Pseudomonas sp. SE83 |