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Literature summary for 3.5.1.93 extracted from

  • Luo, H.; Han, K.; Chang, Y.; Wang, Y.; Tong, S.; Nie, Z.; Wang, X.; Wei, Y.; Yu, H.; Shen, Z.
    Effects of inhibitors on the catalysis and immobilization of cephalosporin C acylase (2018), Chem. Biochem. Eng. Q., 32, 125-133 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme expression in Escherichia coli strain BL21(DE3)/pET-CPCAcy Pseudomonas sp. SE83

Protein Variants

Protein Variants Comment Organism
additional information the recombinant enzyme is immobilized, microenvironment with an acidic pH (about pH 6-7) during the catalysis of cephalosporin C with the immobilized enzyme. The immobilization efficiency is increased when the enzyme is immobilized in the presence of inhibitors sodium acetate and sodium bicarbobate. And the immobilized enzyme, in the presence of inhibitors, has a higher thermostability than the control at 50°C Pseudomonas sp. SE83

Inhibitors

Inhibitors Comment Organism Structure
6-aminopenicillanic acid 6-APA, a noncompetitive inhibitor Pseudomonas sp. SE83
acetate
-
Pseudomonas sp. SE83
bicarbonate
-
Pseudomonas sp. SE83
Butyrate
-
Pseudomonas sp. SE83
ethanolamine
-
Pseudomonas sp. SE83
formate
-
Pseudomonas sp. SE83
glucosamine
-
Pseudomonas sp. SE83
Lactate
-
Pseudomonas sp. SE83
additional information effects of the competitive inhibitors on the catalysis and immobilization of cephalosporin C acylase, mechanism of competitive inhibition, overview Pseudomonas sp. SE83
propionate
-
Pseudomonas sp. SE83
Pyridine
-
Pseudomonas sp. SE83

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cephalosporin C + H2O Pseudomonas sp. SE83
-
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. SE83 P15557
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O
-
Pseudomonas sp. SE83 7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
?

Synonyms

Synonyms Comment Organism
AcyI
-
Pseudomonas sp. SE83
acylase ACY 1 proenzyme UniProt Pseudomonas sp. SE83
CCA
-
Pseudomonas sp. SE83
cephalosporin C acylase
-
Pseudomonas sp. SE83

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
recombinant immobilized enzyme, assay at Pseudomonas sp. SE83
37
-
recombinant free enzyme, assay at Pseudomonas sp. SE83

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
the immobilized enzyme, in the presence of inhibitors, has a higher thermostability than the control. The immobilized enzyme with sodium acetate shows a 31.7% increase in activity and a 25% increase with sodium bicarbonate. The increased stability and activity recovery in the process of immobilization might be attributed to the increase in the enzyme rigidity via binding of inhibitors with CCA at the active sites Pseudomonas sp. SE83

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant free enzyme, assay at Pseudomonas sp. SE83
8.5
-
recombinant immobilized enzyme, assay at Pseudomonas sp. SE83

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Pseudomonas sp. SE83