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Literature summary for 3.5.1.93 extracted from

  • Chang, Y.; Tong, S.; Luo, H.; Liu, Z.; Qin, B.; Zhu, L.; Sun, H.; Yu, H.; Shen, Z.
    Application of ammonium bicarbonate buffer as a smart microenvironmental pH regulator of immobilized cephalosporin C acylase catalysis in different reactors (2019), Biotechnol. Prog., 35, e2846 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information in a stirred tank reactor, during catalysis with immobilized cephalosporin C acylase (CCA), the microenvironmental pH drops to pH 7.2 in a nonbuffered system (with the pH maintained at pH 8.5 by adding alkali) due to the existence of diffusional resistance. The immobilized CCA only catalyzes only five batch reactions, suggesting that the sharp pH gradient impairs the enzyme stability. To buffer the protons produced in the hydrolysis of cephalosporin C by CCA, phosphate and bicarbonate buffers are introduced. When CCA is catalyzed with 0.1 M ammonium bicarbonate buffer, no obvious gradient between the bulk solution and intraparticle pH is detected, and the catalysis of 15 batch reactions is achieved. Accordingly, with 0.2 M ammonium bicarbonate buffer in a packed bed reactor, the immobilized CCA exhibits continuous catalysis with high conversion rates (over 95%) for 21 days. Reactions with ammonium bicarbonate buffer shows significant increases in the stability and catalytic efficiency of the immobilized CCA in different reactors compared to those in nonbuffered systems. Set-up for measurement of internal pH at different positions in the packed-bed reactor, overview Pseudomonas sp. SE83

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. SE83 P15557
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-

Synonyms

Synonyms Comment Organism
AcyI
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Pseudomonas sp. SE83
acylase ACY 1 proenzyme UniProt Pseudomonas sp. SE83
CCA
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Pseudomonas sp. SE83
cephalosporin C acylase
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Pseudomonas sp. SE83

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Pseudomonas sp. SE83

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
immobilized enzyme CCA Pseudomonas sp. SE83

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
the immobilized enzyme is very sensitive to changing pH during the reaction. Proton-forming bioconversion reactions, like the CCA reaction, in pakced-bed reactors, drastic decreases in pH during continuous catalysis have to be avoided Pseudomonas sp. SE83