Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli strain BL21 | Brevundimonas diminuta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Brevundimonas diminuta | |
Mg2+ | - |
Brevundimonas diminuta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O | Brevundimonas diminuta | - |
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | Brevundimonas diminuta NK703 | - |
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | Brevundimonas diminuta | - |
7-aminocephalosporanic acid + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | Brevundimonas diminuta NK703 | - |
7-aminocephalosporanic acid + glutarate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevundimonas diminuta | G3MDB2 | - |
- |
Brevundimonas diminuta NK703 | G3MDB2 | - |
- |
Purification (Comment) | Organism |
---|---|
several chemical treatment techniques commonly used for protein extraction are investigated for recovering glutaryl-7-aminocephalosporanic acid acylase (GLA) from recombinant Escherichia coli cells, best results are obtained by the combined use of 0.5% w/v cetyltrimethylammonium bromide (CTAB) and 0.3 M KCl at 10°C for 12 h, method evaluation and optimization of all extraction parameters, detailed overview | Brevundimonas diminuta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.4 | - |
recombinant enzyme, crude extract, pH 8.0, 37°C | Brevundimonas diminuta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O | - |
Brevundimonas diminuta | 7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Brevundimonas diminuta NK703 | 7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Brevundimonas diminuta | 7-aminocephalosporanic acid + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Brevundimonas diminuta NK703 | 7-aminocephalosporanic acid + glutarate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GLA | - |
Brevundimonas diminuta |
glutaryl-7-aminocephalosporanic acid acylase | - |
Brevundimonas diminuta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Brevundimonas diminuta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Brevundimonas diminuta |