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Literature summary for 3.5.1.93 extracted from

  • Jobanputra, A.; Vasait, R.
    Cephalosporin C acylase from Pseudomonas species production and enhancement of its activity by optimization of process parameters (2015), Biocatal. Agricult. Biotechnol., 4, 465-470 .
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
additional information cephalosporin C acylase has the potential to transform cephalosporin C to 7-aminocephalosporanic acid which is an important intermediate for production of broad-spectrum semisynthetic cephalosporins. Selection of medium components and parameters like temperature, pH, carbon sources, nitrogen sources, and inoculum level for the optimal production of cephalosporin C acylase by Pseudomonas species, overview. The biotransformation is conducted utilizing all optimized parameters for the production of cephalosporin C acylase. The maximum CCA acylase activity is detected at 72 h of incubation. The highest CCA activity for Pseudomonas species of 42.9 U/ml is detected at 72 h of incubation at pH 8.0 and 30°C Pseudomonas sp. D13

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cephalosporin C + H2O Pseudomonas sp. D13
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7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. D13 A0A1D8GRD5 isolated from rhizosphere soil
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O
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Pseudomonas sp. D13 7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
?
additional information the amount of product 7-aminocephalosporanic acid formed is measured by glutaryl p-nitroanilide (GpNA), 4-dimethyl aminobenzaldehyde (p-DAB), and HPLC assays Pseudomonas sp. D13 ?
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-

Synonyms

Synonyms Comment Organism
CCA
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Pseudomonas sp. D13
cephalosporin C acylase
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Pseudomonas sp. D13

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Pseudomonas sp. D13

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas sp. D13