Protein Variants | Comment | Organism |
---|---|---|
additional information | cephalosporin C acylase has the potential to transform cephalosporin C to 7-aminocephalosporanic acid which is an important intermediate for production of broad-spectrum semisynthetic cephalosporins. Selection of medium components and parameters like temperature, pH, carbon sources, nitrogen sources, and inoculum level for the optimal production of cephalosporin C acylase by Pseudomonas species, overview. The biotransformation is conducted utilizing all optimized parameters for the production of cephalosporin C acylase. The maximum CCA acylase activity is detected at 72 h of incubation. The highest CCA activity for Pseudomonas species of 42.9 U/ml is detected at 72 h of incubation at pH 8.0 and 30°C | Pseudomonas sp. D13 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O | Pseudomonas sp. D13 | - |
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. D13 | A0A1D8GRD5 | isolated from rhizosphere soil | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O | - |
Pseudomonas sp. D13 | 7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
additional information | the amount of product 7-aminocephalosporanic acid formed is measured by glutaryl p-nitroanilide (GpNA), 4-dimethyl aminobenzaldehyde (p-DAB), and HPLC assays | Pseudomonas sp. D13 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
CCA | - |
Pseudomonas sp. D13 |
cephalosporin C acylase | - |
Pseudomonas sp. D13 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudomonas sp. D13 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas sp. D13 |