Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, recombinant overexpression of the soluble enzyme in Escherichia coli strain BL21(DE3) using vector pET-28a, optimization of culture consitions in the upscaling to 2.0% w/v lactose inducing in YT medium at 25°C, resulting in over 50fold increased enzyme activity and 14470 U/l and 206 U/l/h, overview | Brevundimonas diminuta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O | Brevundimonas diminuta | - |
(7R)-7-aminocephalosporanate + glutarate | - |
? | |
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O | Brevundimonas diminuta NK703 | - |
(7R)-7-aminocephalosporanate + glutarate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevundimonas diminuta | G3MDB2 | isolated from an oilfield in China | - |
Brevundimonas diminuta NK703 | G3MDB2 | isolated from an oilfield in China | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O | - |
Brevundimonas diminuta | (7R)-7-aminocephalosporanate + glutarate | - |
? | |
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O | - |
Brevundimonas diminuta NK703 | (7R)-7-aminocephalosporanate + glutarate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GLA | - |
Brevundimonas diminuta |
glutaryl-7-aminocephalosporanic acid acylase | - |
Brevundimonas diminuta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Brevundimonas diminuta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Brevundimonas diminuta |