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Literature summary for 3.5.1.93 extracted from

  • Ma, X.; Su, E.; Zhu, Y.; Deng, S.; Wei, D.
    High-level expression of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas diminuta NK703 in Escherichia coli by combined optimization strategies (2013), J. Biotechnol., 168, 607-615.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, recombinant overexpression of the soluble enzyme in Escherichia coli strain BL21(DE3) using vector pET-28a, optimization of culture consitions in the upscaling to 2.0% w/v lactose inducing in YT medium at 25°C, resulting in over 50fold increased enzyme activity and 14470 U/l and 206 U/l/h, overview Brevundimonas diminuta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O Brevundimonas diminuta
-
(7R)-7-aminocephalosporanate + glutarate
-
?
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O Brevundimonas diminuta NK703
-
(7R)-7-aminocephalosporanate + glutarate
-
?

Organism

Organism UniProt Comment Textmining
Brevundimonas diminuta G3MDB2 isolated from an oilfield in China
-
Brevundimonas diminuta NK703 G3MDB2 isolated from an oilfield in China
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
-
Brevundimonas diminuta (7R)-7-aminocephalosporanate + glutarate
-
?
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
-
Brevundimonas diminuta NK703 (7R)-7-aminocephalosporanate + glutarate
-
?

Synonyms

Synonyms Comment Organism
GLA
-
Brevundimonas diminuta
glutaryl-7-aminocephalosporanic acid acylase
-
Brevundimonas diminuta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Brevundimonas diminuta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Brevundimonas diminuta