Protein Variants | Comment | Organism |
---|---|---|
H296S/H309S | the mutant also shows activity with cephalosporin C (3fold increase compared to the wild type enzyme) | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutaryl-7-aminocephalosporanic acid | substrate inhibition | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | wild type enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
1 | - |
glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid | wild type enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
1.3 | - |
glutaryl-7-aminodesacetoxycephalosporanic acid | wild type enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
1.5 | - |
glutaryl-7-aminodesacetoxycephalosporanic acid | recombinant enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
1.5 | - |
glutaryl-7-aminocephalosporanic acid | wild type enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
2.2 | - |
glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid | recombinant enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
2.5 | - |
glutaryl-7-aminocephalosporanic acid | wild type enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
2.8 | - |
succinyl-7-aminocephalosporanic acid | recombinant enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
2.8 | - |
succinyl-7-aminocephalosporanic acid | wild type enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
3 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme H296S/H309S, at pH 8.0 and 25°C | Pseudomonas sp. | |
3.3 | - |
glutaryl-7-aminocephalosporanic acid | recombinant enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
3.8 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | recombinant enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
4.3 | - |
adipyl-7-aminocephalosporanic acid | mutant enzyme H296S/H309S, at pH 8.0 and 25°C | Pseudomonas sp. | |
4.4 | - |
adipyl-7-aminocephalosporanic acid | recombinant enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
5.4 | - |
succinyl-7-aminocephalosporanic acid | mutant enzyme H296S/H309S, at pH 8.0 and 25°C | Pseudomonas sp. | |
5.4 | - |
glutaryl-7-aminocephalosporanic acid | recombinant enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
6.9 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme H296S/H309S, at pH 8.0 and 25°C | Pseudomonas sp. | |
7.5 | - |
adipyl-7-aminocephalosporanic acid | wild type enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
7.9 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme H296S/H309S, at pH 8.0 and 25°C | Pseudomonas sp. | |
8.7 | - |
glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid | mutant enzyme H296S/H309S, at pH 8.0 and 25°C | Pseudomonas sp. | |
9.5 | - |
cephalosporin C | wild type enzyme, at pH 8.0 and 25°C | Pseudomonas sp. | |
12.2 | - |
cephalosporin C | mutant enzyme H296S/H309S, at pH 8.0 and 25°C | Pseudomonas sp. | |
14.1 | - |
glutaryl-7-aminodesacetoxycephalosporanic acid | mutant enzyme H296S/H309S, at pH 8.0 and 25°C | Pseudomonas sp. | |
16.1 | - |
pentanoyl-7-aminocephalosporanic acid | mutant enzyme H296S/H309S, at pH 8.0 and 25°C | Pseudomonas sp. | |
17.4 | - |
pentanoyl-7-aminocephalosporanic acid | wild type enzyme, at pH 8.0 and 25°C | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adipyl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. | adipate + 7-aminocephalosporanate | - |
? | |
adipyl-7-aminodesacetoxycephalosporanic acid + H2O | 57.4% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. | adipate + 7-aminodesacetoxycephalosporanate | - |
? | |
cephalosporin C + H2O | - |
Pseudomonas sp. | cephalosporamic acid + 2-amino-5-hydroxypentanoate | - |
? | |
glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid + H2O | 81.9% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. | glutarate + 3-chloro-7-aminodesacetoxycephalosporanate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | 100% activity | Pseudomonas sp. | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | 9.9% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. | glutarate + 7-aminocephalosporanate | - |
? | |
glutaryl-7-aminodesacetoxycephalosporanic acid + H2O | 71.5% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. | glutarate + 7-aminodesacetoxycephalosporanate | - |
? | |
glutaryl-D-Phe-Gly + H2O | about 25% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. | glutarate + D-Phe-Gly | - |
? | |
glutaryl-glycine + H2O | less than 5% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. | glutarate + glycine | - |
? | |
glutaryl-L-Ala + H2O | about 25% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. | glutarate + L-Ala | - |
? | |
glutaryl-L-Phe + H2O | about 10% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. | glutarate + L-Phe | - |
? | |
glutaryl-L-Phe-Gly + H2O | about 50% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. | glutarate + L-Phe-Gly | - |
? | |
additional information | the recombinant enzyme shows no activity towards cephalosporin C, butanoyl-7-aminodesacetoxycephalosporanic acid and pentanoyl-7-aminodesacetoxycephalosporanic acid | Pseudomonas sp. | ? | - |
? | |
pentanoyl-7-aminocephalosporanic acid + H2O | about 2.4% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. | pentanoate + 7-aminocephalosporanate | - |
? | |
succinyl-7-aminocephalosporanic acid + H2O | 25.8% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. | succinate + 7-aminocephalosporanate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GAR | commercial preparation | Pseudomonas sp. |
glutaryl acylase | - |
Pseudomonas sp. |
glutaryl-7-ACA acylase | - |
Pseudomonas sp. |
VAC | - |
Pseudomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
glutaryl-7-aminocephalosporanic acid | wild type enzyme, at pH 8.0 and 25°C | Pseudomonas sp. |