Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain DH10B | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
F375L | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
F375M | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
N266H | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
N266M | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
N266Q | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
N266Q/F375L | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
Y178F/F375H | the mutation synergistically improve the catalytic efficiency towards adipyl-7-ADCA 36fold, The activity of this double mutant towards adipyl-7-ADCA is 50% of the activity of the wild-type enzyme towards the preferred substrate glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. |
Y178H | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
Y178H/N266H | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
Y178H/N266M | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
Y178H/N266Q/F375L | decreased activity compared to the wild type enzyme | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266H | Pseudomonas sp. | |
0.042 | - |
glutaryl-7-aminocephalosporanic acid | wild type enzyme | Pseudomonas sp. | |
0.08 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H | Pseudomonas sp. | |
0.08 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H/N266H | Pseudomonas sp. | |
0.08 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H/N266Q/F375L | Pseudomonas sp. | |
0.12 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266Q | Pseudomonas sp. | |
0.14 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266H | Pseudomonas sp. | |
0.17 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266M | Pseudomonas sp. | |
0.17 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme F375M | Pseudomonas sp. | |
0.17 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266Q/F375L | Pseudomonas sp. | |
0.17 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266Q/F375L | Pseudomonas sp. | |
0.17 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H/N266M | Pseudomonas sp. | |
0.19 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H/N266H | Pseudomonas sp. | |
0.26 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme F375L | Pseudomonas sp. | |
0.28 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H/N266Q/F375L | Pseudomonas sp. | |
0.3 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H/N266M | Pseudomonas sp. | |
0.33 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266M | Pseudomonas sp. | |
0.47 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H | Pseudomonas sp. | |
0.62 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266Q | Pseudomonas sp. | |
0.65 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme F375M | Pseudomonas sp. | |
0.7 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme F375L | Pseudomonas sp. | |
0.8 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | wild type enzyme | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. SY-77 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adipyl-7-aminodesacetoxycephalosporanic acid + H2O | - |
Pseudomonas sp. | ? | - |
? | |
adipyl-7-aminodesacetoxycephalosporanic acid + H2O | - |
Pseudomonas sp. SY-77 | ? | - |
? | |
cephalosporin C + H2O | - |
Pseudomonas sp. | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Pseudomonas sp. SY-77 | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. SY-77 | 7-aminocephalosporanate + glutarate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adipyl-cephalosporin acylase | - |
Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | wild type enzyme | Pseudomonas sp. | |
0.46 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266Q | Pseudomonas sp. | |
0.47 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266H | Pseudomonas sp. | |
0.48 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266Q/F375L | Pseudomonas sp. | |
0.65 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme F375M | Pseudomonas sp. | |
0.66 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H | Pseudomonas sp. | |
0.66 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H/N266Q/F375L | Pseudomonas sp. | |
0.67 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme F375L | Pseudomonas sp. | |
0.75 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266Q/F375L | Pseudomonas sp. | |
0.89 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H/N266M | Pseudomonas sp. | |
0.95 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H/N266H | Pseudomonas sp. | |
1.1 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme F375L | Pseudomonas sp. | |
1.2 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme Y178H/N266M | Pseudomonas sp. | |
1.8 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266M | Pseudomonas sp. | |
1.9 | - |
adipyl-7-aminodesacetoxycephalosporanic acid | mutant enzyme N266M | Pseudomonas sp. | |
2.02 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme F375M | Pseudomonas sp. | |
2.2 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H/N266H | Pseudomonas sp. | |
2.4 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266Q | Pseudomonas sp. | |
2.5 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H | Pseudomonas sp. | |
2.5 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme Y178H/N266Q/F375L | Pseudomonas sp. | |
3.1 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266H | Pseudomonas sp. | |
4.1 | - |
glutaryl-7-aminocephalosporanic acid | wild type enzyme | Pseudomonas sp. |