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Literature summary for 3.5.1.93 extracted from

  • Zheng, H.; Chen, J.; Su, L.; Zhao, Y.; Yang, Y.; Zeng, H.Y.; Xu, G.; Yang, S.; Jiang, W.
    One-step purification and immobilization of his-tagged GL-7-ACA acylase (2007), Enzyme Microb. Technol., 41, 474-479.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3)/pET28GA01 cells Pseudomonas sp.

General Stability

General Stability Organism
FP-IDA-Ni2+ resins-immobilized GLA is recycled 21times without significant loss of activity Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
1 * 54000 + 1 * 16000, SDS-PAGE Pseudomonas sp.
54000
-
1 * 54000 + 1 * 16000, SDS-PAGE Pseudomonas sp.
70000
-
SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. O86089
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON affinity resin column chromatography Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaryl-7-aminocephalosporanic acid + H2O
-
Pseudomonas sp. 7-aminocephalosporanate + glutarate
-
?

Subunits

Subunits Comment Organism
dimer 1 * 54000 + 1 * 16000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
cephalosporin acylase
-
Pseudomonas sp.
GL-7-ACA acylase
-
Pseudomonas sp.
GLA
-
Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
immobilized GLA shows good stability at temperature up to 30°C Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 11 immobilized GLA shows good stability at pH value below 11.0 Pseudomonas sp.