Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Glutaric acid | inducer for cephalosporin acylase synthesis | Pseudomonas sp. | |
Glutaric acid | inducer for cephalosporin acylase synthesis | Pseudomonas putida | |
Glutaric acid | inducer for cephalosporin acylase synthesis | Brevibacillus laterosporus | |
Glutaric acid | inducer for cephalosporin acylase synthesis | Sphingomonas paucimobilis | |
Glutaric acid | inducer for cephalosporin acylase synthesis | Aeromonas sp. | |
Glutaric acid | inducer for cephalosporin acylase synthesis | Flavobacterium sp. | |
Glutaric acid | inducer for cephalosporin acylase synthesis | Brevundimonas diminuta | |
Glutaric acid | inducer for cephalosporin acylase synthesis | Achromobacter xylosoxidans | |
Glutaric acid | inducer for cephalosporin acylase synthesis | Pseudomonas nitroreducens | |
Glutaric acid | inducer for cephalosporin acylase synthesis | Rhizobium viscosum | |
monopotassium anthroquinone 1,8-di-sulfonate | - |
Paecilomyces sp. (in: Eurotiomycetes) | |
N-isobutoxycarbomyl cephalosporin C | - |
Paecilomyces sp. (in: Eurotiomycetes) |
Cloned (Comment) | Organism |
---|---|
expressed in Bacillus subtilis and Escherichia coli strain JM 109 | Brevibacillus laterosporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
glutaryl-7-aminocephalosporanic acid | - |
Pseudomonas sp. | |
2.6 | - |
glutaryl-7-aminocephalosporanic acid | - |
Brevundimonas diminuta | |
3.2 | - |
glutaryl-7-aminocephalosporanic acid | - |
Brevibacillus laterosporus | |
4.8 | - |
cephalosporin C | - |
Brevundimonas diminuta | |
10.41 | - |
glutaryl-7-aminocephalosporanic acid | - |
Pseudomonas nitroreducens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
1 * 35000 + 1 * 21000, SDS-PAGE | Pseudomonas nitroreducens |
26000 | - |
1 * 58000 + 1 * 26000, SDS-PAGE | Brevundimonas diminuta |
35000 | - |
1 * 35000 + 1 * 21000, SDS-PAGE | Pseudomonas nitroreducens |
58000 | - |
SDS-PAGE | Pseudomonas nitroreducens |
58000 | - |
1 * 58000 + 1 * 26000, SDS-PAGE | Brevundimonas diminuta |
61000 | - |
1 * 61000 + 1* 28000, SDS-PAGE | Pseudomonas sp. |
70000 | - |
SDS-PAGE | Brevibacillus laterosporus |
84000 | - |
SDS-PAGE | Brevundimonas diminuta |
89000 | - |
SDS-PAGE | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter xylosoxidans | - |
- |
- |
Aeromonas sp. | - |
- |
- |
Aeromonas sp. ACY 95 | - |
- |
- |
Arthrobacter sp. | - |
- |
- |
Arthrobacter sp. 5-8A | - |
- |
- |
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) SV12 | - |
- |
- |
Brevibacillus laterosporus | - |
- |
- |
Brevibacillus laterosporus J1 | - |
- |
- |
Brevundimonas diminuta | - |
- |
- |
Brevundimonas diminuta N 176 | - |
- |
- |
Burkholderia cepacia | - |
- |
- |
Burkholderia cepacia BY21 | - |
- |
- |
Flavobacterium sp. | - |
- |
- |
Flavobacterium sp. 650 | - |
- |
- |
Paecilomyces sp. (in: Eurotiomycetes) | - |
- |
- |
Pseudomonas nitroreducens | - |
- |
- |
Pseudomonas nitroreducens CCRC 11041 | - |
- |
- |
Pseudomonas putida | - |
strain ATCC 950 | - |
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. A14 | - |
- |
- |
Pseudomonas sp. BLO 72 | - |
- |
- |
Rhizobium viscosum | - |
- |
- |
Sphingomonas paucimobilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, TEAE-cellulose chromatography, Toyopearl HW65F column chromatography, CM Toyopearl 650 column chromatography, Sephadex G-200 gel filtration, Q-Sepharose column chromatography, hydroxyapatite column chromatography, SP-Trisacryl gel filtration, and Sephacryl S-300gel filtration | Arthrobacter sp. |
ammonium sulfate precipitation, Toyopearl HW65F column chromatography, and CM Toyopearl 650 column chromatography | Brevibacillus laterosporus |
ammonium sulfate precipitation, Toyopearl HW65F column chromatography, and CM Toyopearl 650 column chromatography | Brevundimonas diminuta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O | - |
Pseudomonas sp. | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Pseudomonas putida | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Sphingomonas paucimobilis | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Aeromonas sp. | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Flavobacterium sp. | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Brevundimonas diminuta | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Achromobacter xylosoxidans | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Rhizobium viscosum | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Aeromonas sp. ACY 95 | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Flavobacterium sp. 650 | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Pseudomonas sp. A14 | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Pseudomonas sp. BLO 72 | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Brevundimonas diminuta N 176 | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas putida | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Bacillus sp. (in: Bacteria) | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Arthrobacter sp. | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Brevibacillus laterosporus | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Sphingomonas paucimobilis | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Burkholderia cepacia | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Aeromonas sp. | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Flavobacterium sp. | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Brevundimonas diminuta | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas nitroreducens | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Rhizobium viscosum | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Paecilomyces sp. (in: Eurotiomycetes) | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Aeromonas sp. ACY 95 | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Burkholderia cepacia BY21 | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Arthrobacter sp. 5-8A | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Flavobacterium sp. 650 | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. A14 | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Brevibacillus laterosporus J1 | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas nitroreducens CCRC 11041 | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Bacillus sp. (in: Bacteria) SV12 | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. BLO 72 | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Brevundimonas diminuta N 176 | 7-aminocephalosporanate + glutarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 35000 + 1 * 21000, SDS-PAGE | Pseudomonas nitroreducens |
dimer | 1 * 58000 + 1 * 26000, SDS-PAGE | Brevundimonas diminuta |
dimer | 1 * 61000 + 1* 28000, SDS-PAGE | Pseudomonas sp. |
monomer | - |
Brevibacillus laterosporus |
Synonyms | Comment | Organism |
---|---|---|
cephalosporin acylase | - |
Pseudomonas sp. |
cephalosporin acylase | - |
Pseudomonas putida |
cephalosporin acylase | - |
Sphingomonas paucimobilis |
cephalosporin acylase | - |
Aeromonas sp. |
cephalosporin acylase | - |
Flavobacterium sp. |
cephalosporin acylase | - |
Brevundimonas diminuta |
cephalosporin acylase | - |
Rhizobium viscosum |
cephalosporin acylase | - |
Paecilomyces sp. (in: Eurotiomycetes) |
cephalosporin acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Achromobacter xylosoxidans |
glutaryl-7-ACA acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Pseudomonas sp. |
glutaryl-7-ACA acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Pseudomonas putida |
glutaryl-7-ACA acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Bacillus sp. (in: Bacteria) |
glutaryl-7-ACA acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Arthrobacter sp. |
glutaryl-7-ACA acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Brevibacillus laterosporus |
glutaryl-7-ACA acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Sphingomonas paucimobilis |
glutaryl-7-ACA acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Burkholderia cepacia |
glutaryl-7-ACA acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Aeromonas sp. |
glutaryl-7-ACA acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Flavobacterium sp. |
glutaryl-7-ACA acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Brevundimonas diminuta |
glutaryl-7-ACA acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Achromobacter xylosoxidans |
glutaryl-7-ACA acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Pseudomonas nitroreducens |
glutaryl-7-ACA acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Rhizobium viscosum |
glutaryl-7-ACA acylase | glutaryl-7-ACA acylases and cephalosporin acylases have been collectively referred to as cephalosporin acylases because they all have either glutaryl-7-aminocephalosporanic acid or cephalosporin C as their substrates | Paecilomyces sp. (in: Eurotiomycetes) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Brevibacillus laterosporus |
43 | - |
- |
Pseudomonas nitroreducens |
48 | - |
- |
Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
9 | - |
the enzyme activity sharply drops on either side of pH 9 | Brevibacillus laterosporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Pseudomonas nitroreducens |
9 | - |
- |
Brevibacillus laterosporus |
9 | - |
- |
Brevundimonas diminuta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | - |
Pseudomonas sp. |
8 | 10 | - |
Brevundimonas diminuta |