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Literature summary for 3.5.1.93 extracted from

  • Khatuntseva, S.A.; Eldarov, M.A.; Lopatin, S.A.; Zeinalov, O.A.; Skryabin, K.G.
    Cloning and expression of variants of the glutaryl-7-aminocephalosporic acid acylase of the bacterium Brevundimonas diminuta in Escherichia coli cells (2007), Appl. Biochem. Microbiol., 43, 414-421.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Brevundimonas diminuta

Organism

Organism UniProt Comment Textmining
Brevundimonas diminuta
-
-
-
Brevundimonas diminuta VKM B-1297
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, chitin granula column chromatography, and Co2+-iminodiacetic acid-Sepharose column chromatography Brevundimonas diminuta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
crude extract, at 37°C Brevundimonas diminuta
10
-
after 6fold purification, at 37°C Brevundimonas diminuta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O
-
Brevundimonas diminuta cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
?
cephalosporin C + H2O
-
Brevundimonas diminuta VKM B-1297 cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
?
glutaryl-7-aminocephalosporanic acid + H2O
-
Brevundimonas diminuta 7-aminocephalosporanate + glutarate
-
?
glutaryl-7-aminocephalosporanic acid + H2O
-
Brevundimonas diminuta VKM B-1297 7-aminocephalosporanate + glutarate
-
?

Subunits

Subunits Comment Organism
More the enzym is composed of 54 kDa alpha-subunits and 20 kDa beta-subunits Brevundimonas diminuta

Synonyms

Synonyms Comment Organism
Gl7ACA acylase
-
Brevundimonas diminuta
glutaryl-7-aminocephalosporic acid acylase
-
Brevundimonas diminuta