Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Brevundimonas diminuta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevundimonas diminuta | - |
- |
- |
Brevundimonas diminuta VKM B-1297 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, chitin granula column chromatography, and Co2+-iminodiacetic acid-Sepharose column chromatography | Brevundimonas diminuta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
crude extract, at 37°C | Brevundimonas diminuta |
10 | - |
after 6fold purification, at 37°C | Brevundimonas diminuta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O | - |
Brevundimonas diminuta | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | - |
Brevundimonas diminuta VKM B-1297 | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Brevundimonas diminuta | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Brevundimonas diminuta VKM B-1297 | 7-aminocephalosporanate + glutarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzym is composed of 54 kDa alpha-subunits and 20 kDa beta-subunits | Brevundimonas diminuta |
Synonyms | Comment | Organism |
---|---|---|
Gl7ACA acylase | - |
Brevundimonas diminuta |
glutaryl-7-aminocephalosporic acid acylase | - |
Brevundimonas diminuta |