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Literature summary for 3.5.1.93 extracted from

  • Kim, J.K.; Yang, I.S.; Shin, H.J.; Cho, K.J.; Ryu, E.K.; Kim, S.H.; Park, S.S.; Kim, K.H.
    Insight into autoproteolytic activation from the structure of cephalosporin acylase: a protein with two proteolytic chemistries (2006), Proc. Natl. Acad. Sci. USA, 103, 1732-1737.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21/DE3 Pseudomonas sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion method, mutant enzymes Y202L, R226K, S170C and E159Q Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. A4ZVL3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme is processed in two sequential steps of intramolecular autoproteolysis involving two distinct proteolytic mechanisms, the first mediatzed by a serine residue and the second by a glutamate Pseudomonas sp.

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Synonyms

Synonyms Comment Organism
cephalosporin acylase
-
Pseudomonas sp.