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Literature summary for 3.5.1.93 extracted from

  • Yau, M.H.; Wang, J.; Tsang, P.W.; Fong, W.P.
    J1 acylase, a glutaryl-7-aminocephalosporanic acid acylase from Bacillus laterosporus J1, is a member of the alpha/beta-hydrolase fold superfamily (2006), FEBS Lett., 580, 1465-1471.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Brevibacillus laterosporus

Protein Variants

Protein Variants Comment Organism
D264A no hydrolysis of glutaryl-7-aminocephalosporanic acid Brevibacillus laterosporus
H309A no hydrolysis of glutaryl-7-aminocephalosporanic acid Brevibacillus laterosporus
S125A no hydrolysis of glutaryl-7-aminocephalosporanic acid Brevibacillus laterosporus
W173A kcat/KM is 2.1fold lower than wild-type value Brevibacillus laterosporus
Y126A kcat/KM is 9.2 fold lower than wild-type value Brevibacillus laterosporus
Y126F kcat/KM is identical to wild-type value Brevibacillus laterosporus
Y57A no hydrolysis of glutaryl-7-aminocephalosporanic acid Brevibacillus laterosporus
Y57F no hydrolysis of glutaryl-7-aminocephalosporanic acid Brevibacillus laterosporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 37°C, mutant enzyme Y126F Brevibacillus laterosporus
0.19
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 37°C, wild-type enzyme Brevibacillus laterosporus
1.2
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 37°C, mutant enzyme W173A Brevibacillus laterosporus
1.69
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 37°C, mutant enzyme Y126A Brevibacillus laterosporus

Organism

Organism UniProt Comment Textmining
Brevibacillus laterosporus
-
-
-
Brevibacillus laterosporus J1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Brevibacillus laterosporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaryl-7-aminocephalosporanic acid + H2O
-
Brevibacillus laterosporus 7-aminocephalosporanate + glutarate
-
?
glutaryl-7-aminocephalosporanic acid + H2O
-
Brevibacillus laterosporus J1 7-aminocephalosporanate + glutarate
-
?

Synonyms

Synonyms Comment Organism
J1 acylase
-
Brevibacillus laterosporus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 37°C, mutant enzyme Y126F Brevibacillus laterosporus
0.031 0.51 glutaryl-7-aminocephalosporanic acid pH 8.0, 37°C, wild-type enzyme Brevibacillus laterosporus
0.23
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 37°C, mutant enzyme W173A Brevibacillus laterosporus
6.05
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 37°C, mutant enzyme Y126F Brevibacillus laterosporus
7.16
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 37°C, mutant enzyme Y126A Brevibacillus laterosporus
7.54
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 37°C, wild-type enzyme Brevibacillus laterosporus