Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Brevibacillus laterosporus |
Protein Variants | Comment | Organism |
---|---|---|
D264A | no hydrolysis of glutaryl-7-aminocephalosporanic acid | Brevibacillus laterosporus |
H309A | no hydrolysis of glutaryl-7-aminocephalosporanic acid | Brevibacillus laterosporus |
S125A | no hydrolysis of glutaryl-7-aminocephalosporanic acid | Brevibacillus laterosporus |
W173A | kcat/KM is 2.1fold lower than wild-type value | Brevibacillus laterosporus |
Y126A | kcat/KM is 9.2 fold lower than wild-type value | Brevibacillus laterosporus |
Y126F | kcat/KM is identical to wild-type value | Brevibacillus laterosporus |
Y57A | no hydrolysis of glutaryl-7-aminocephalosporanic acid | Brevibacillus laterosporus |
Y57F | no hydrolysis of glutaryl-7-aminocephalosporanic acid | Brevibacillus laterosporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 37°C, mutant enzyme Y126F | Brevibacillus laterosporus | |
0.19 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 37°C, wild-type enzyme | Brevibacillus laterosporus | |
1.2 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 37°C, mutant enzyme W173A | Brevibacillus laterosporus | |
1.69 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 37°C, mutant enzyme Y126A | Brevibacillus laterosporus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacillus laterosporus | - |
- |
- |
Brevibacillus laterosporus J1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Brevibacillus laterosporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-7-aminocephalosporanic acid + H2O | - |
Brevibacillus laterosporus | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Brevibacillus laterosporus J1 | 7-aminocephalosporanate + glutarate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
J1 acylase | - |
Brevibacillus laterosporus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 37°C, mutant enzyme Y126F | Brevibacillus laterosporus | |
0.031 | 0.51 | glutaryl-7-aminocephalosporanic acid | pH 8.0, 37°C, wild-type enzyme | Brevibacillus laterosporus | |
0.23 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 37°C, mutant enzyme W173A | Brevibacillus laterosporus | |
6.05 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 37°C, mutant enzyme Y126F | Brevibacillus laterosporus | |
7.16 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 37°C, mutant enzyme Y126A | Brevibacillus laterosporus | |
7.54 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 37°C, wild-type enzyme | Brevibacillus laterosporus |