Protein Variants | Comment | Organism |
---|---|---|
E455D | the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 4.5fold lower than the wild-type ratio | Pseudomonas sp. 130 |
E455L | the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 34fold lower than the wild-type ratio | Pseudomonas sp. 130 |
E455Q | the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 6.1fold lower than the wild-type ratio | Pseudomonas sp. 130 |
H23D | the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 9.7fold lower than to the wild-type ratio | Pseudomonas sp. 130 |
H23Q | the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 4.3fold lower than the wild-type ratio | Pseudomonas sp. 130 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 30°C, wild-type enzyme | Pseudomonas sp. 130 | |
0.82 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 30°C, beta-subunitmutant enzyme H23Q | Pseudomonas sp. 130 | |
1.18 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 30°C, beta-subunitmutant enzyme E455Q | Pseudomonas sp. 130 | |
1.24 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 30°C, beta-subunitmutant enzyme E455D | Pseudomonas sp. 130 | |
1.76 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 30°C, beta-subunitmutant enzyme H23D | Pseudomonas sp. 130 | |
3.1 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 30°C, beta-subunit mutant enzyme E455L | Pseudomonas sp. 130 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. 130 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | His23beta is essential for autoproteolysis, Glu455beta is responsible for the efficiency of the process | Pseudomonas sp. 130 |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. 130 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13 | - |
- |
Pseudomonas sp. 130 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. 130 | 7-aminocephalosporanate + glutarate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutaryl-7-amino cephalosporanic acid acylase | - |
Pseudomonas sp. 130 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
mutant enzyme E455L is unstable at | Pseudomonas sp. 130 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 30°C, beta-subunitmutant enzyme E455L | Pseudomonas sp. 130 | |
6.1 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 30°C, beta-subunitmutant enzyme H23D | Pseudomonas sp. 130 | |
6.5 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 30°C, beta-subunit mutant enzyme H23Q | Pseudomonas sp. 130 | |
6.6 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 30°C, beta-subunitmutant enzyme E455Q | Pseudomonas sp. 130 | |
9.4 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 30°C, beta-subunitmutant enzyme E455D | Pseudomonas sp. 130 | |
16.9 | - |
glutaryl-7-aminocephalosporanic acid | pH 8.0, 30°C, wild-type enzyme | Pseudomonas sp. 130 |