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Literature summary for 3.5.1.93 extracted from

  • Mao, X.; Wang, W.; Jiang, W.; Zhao, G.P.
    His23beta and Glu455beta of the Pseudomonas sp. 130 glutaryl-7-amino cephalosporanic acid acylase are crucially important for efficient autoproteolysis and enzymatic catalysis (2004), Protein J., 23, 197-204.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E455D the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 4.5fold lower than the wild-type ratio Pseudomonas sp. 130
E455L the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 34fold lower than the wild-type ratio Pseudomonas sp. 130
E455Q the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 6.1fold lower than the wild-type ratio Pseudomonas sp. 130
H23D the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 9.7fold lower than to the wild-type ratio Pseudomonas sp. 130
H23Q the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 4.3fold lower than the wild-type ratio Pseudomonas sp. 130

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 30°C, wild-type enzyme Pseudomonas sp. 130
0.82
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 30°C, beta-subunitmutant enzyme H23Q Pseudomonas sp. 130
1.18
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 30°C, beta-subunitmutant enzyme E455Q Pseudomonas sp. 130
1.24
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 30°C, beta-subunitmutant enzyme E455D Pseudomonas sp. 130
1.76
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 30°C, beta-subunitmutant enzyme H23D Pseudomonas sp. 130
3.1
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 30°C, beta-subunit mutant enzyme E455L Pseudomonas sp. 130

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. 130
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification His23beta is essential for autoproteolysis, Glu455beta is responsible for the efficiency of the process Pseudomonas sp. 130

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp. 130

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13
-
-
Pseudomonas sp. 130

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaryl-7-aminocephalosporanic acid + H2O
-
Pseudomonas sp. 130 7-aminocephalosporanate + glutarate
-
?

Synonyms

Synonyms Comment Organism
glutaryl-7-amino cephalosporanic acid acylase
-
Pseudomonas sp. 130

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
mutant enzyme E455L is unstable at Pseudomonas sp. 130

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 30°C, beta-subunitmutant enzyme E455L Pseudomonas sp. 130
6.1
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 30°C, beta-subunitmutant enzyme H23D Pseudomonas sp. 130
6.5
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 30°C, beta-subunit mutant enzyme H23Q Pseudomonas sp. 130
6.6
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 30°C, beta-subunitmutant enzyme E455Q Pseudomonas sp. 130
9.4
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 30°C, beta-subunitmutant enzyme E455D Pseudomonas sp. 130
16.9
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 30°C, wild-type enzyme Pseudomonas sp. 130