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Literature summary for 3.5.1.93 extracted from

  • Li, Y.; Jiang, W.; Yang, Y.; Zhao, G.; Wang, E.
    Overproduction and purification of glutaryl 7-amino cephalosporanic acid acylase (1998), Protein Expr. Purif., 12, 233-238.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp. 130

Protein Variants

Protein Variants Comment Organism
additional information deletion of the signal peptide and mutation in the alpha-subunit of the acylase has little influence on its posttranslational processing and its kinetic parameters Pseudomonas sp. 130

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46
-
glutaryl-7-aminocephalosporanic acid
-
Pseudomonas sp. 130

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. 130
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp. 130

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
-
Pseudomonas sp. 130

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaryl-7-aminocephalosporanic acid + H2O
-
Pseudomonas sp. 130 7-aminocephalosporanate + glutarate
-
?

Synonyms

Synonyms Comment Organism
GL-7ACA acylase
-
Pseudomonas sp. 130
glutaryl 7-amino cephalosporanic acid acylase
-
Pseudomonas sp. 130