Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7-aminocephalosporanate | competitive | Brevibacillus laterosporus | |
7-aminocephalosporanate | competitive | Brevundimonas diminuta | |
7-aminocephalosporanate | competitive | Pseudomonas sp. | |
EDTA | 5 mM, 27% inhibition | Brevundimonas diminuta | |
Glutarate | competitive | Brevibacillus laterosporus | |
Glutarate | competitive | Brevundimonas diminuta | |
Glutarate | competitive | Pseudomonas sp. | |
PCMB | 92% inhibition at 1 mM, 96% inhibition at 1 mM | Brevundimonas diminuta | |
PCMB | 5 mM, 10% inhibition | Pseudomonas sp. | |
PMSF | 10% inhibition at 1 mM, 22% inhibition at 5 mM | Brevibacillus laterosporus | |
PMSF | 5 mM, 38% inhibition | Brevundimonas diminuta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
glutaryl-7-aminocephalosporanic acid | - |
Pseudomonas sp. | |
2.6 | - |
glutaryl-7-aminocephalosporanic acid | - |
Brevundimonas diminuta | |
3.2 | - |
glutaryl-7-aminocephalosporanic acid | - |
Brevibacillus laterosporus | |
4.8 | - |
cephalosporin C | - |
Brevundimonas diminuta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
1 * 26000 + 1 * 58000, SDS-PAGE | Brevundimonas diminuta |
28000 | - |
1 * 28000 + 1 * 61000, SDS-PAGE | Pseudomonas sp. |
50000 | - |
gel filtration | Brevibacillus laterosporus |
54000 | - |
gel filtration | Pseudomonas sp. |
54000 | - |
gel filtration | Brevundimonas diminuta |
58000 | - |
1 * 26000 + 1 * 58000, SDS-PAGE | Brevundimonas diminuta |
61000 | - |
1 * 28000 + 1 * 61000, SDS-PAGE | Pseudomonas sp. |
70000 | - |
1 * 70000, SDS-PAGE | Brevibacillus laterosporus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacillus laterosporus | - |
J1 | - |
Brevibacillus laterosporus J1 | - |
J1 | - |
Brevundimonas diminuta | - |
N176 | - |
Brevundimonas diminuta N176 | - |
N176 | - |
Pseudomonas sp. | - |
A14, recombinant enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pseudomonas sp. |
recombinant enzyme | Brevibacillus laterosporus |
recombinant enzyme | Brevundimonas diminuta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.3 | - |
- |
Brevibacillus laterosporus |
7.1 | - |
- |
Pseudomonas sp. |
100 | - |
- |
Brevundimonas diminuta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adipyl-7-aminocephalosporanic acid + H2O | activity is 19% of the activity with glutaryl-7-aminocephalosporanic acid | Brevundimonas diminuta | 7-aminocephalosporanate + adipate | - |
? | |
adipyl-7-aminocephalosporanic acid + H2O | activity is 19% of the activity with glutaryl-7-aminocephalosporanic acid | Brevundimonas diminuta N176 | 7-aminocephalosporanate + adipate | - |
? | |
adipyl-7-aminocephalosporanic acid + H2O | activity is 0.56% of the activity with glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. | 7-aminocephalosporanic acid + adipate | - |
? | |
adipyl-7-aminocephalosporanic acid + H2O | activity is 68% of the activity with glutaryl-7-aminocephalosporanic acid | Brevibacillus laterosporus | 7-aminocephalosporanic acid + adipate | - |
? | |
adipyl-7-aminocephalosporanic acid + H2O | activity is 68% of the activity with glutaryl-7-aminocephalosporanic acid | Brevibacillus laterosporus J1 | 7-aminocephalosporanic acid + adipate | - |
? | |
cephalosporin C + H2O | weak activity | Pseudomonas sp. | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | weak activity | Brevibacillus laterosporus | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | activity is 4% of the activity with glutaryl-7-aminocephalosporanic acid | Brevundimonas diminuta | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | activity is 4% of the activity with glutaryl-7-aminocephalosporanic acid | Brevundimonas diminuta N176 | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | weak activity | Brevibacillus laterosporus J1 | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
D-glutamyl-7-aminocephalosporanic acid + H2O | activity is 0.85% of the activity with glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. | 7-aminocephalosporanic acid + D-glutamate | - |
? | |
D-glutamyl-7-aminocephalosporanic acid + H2O | activity is 0.55% of the activity with glutaryl-7-aminocephalosporanic acid | Brevibacillus laterosporus | 7-aminocephalosporanic acid + glutamate | - |
? | |
D-glutamyl-7-aminocephalosporanic acid + H2O | activity is 0.55% of the activity with glutaryl-7-aminocephalosporanic acid | Brevibacillus laterosporus J1 | 7-aminocephalosporanic acid + glutamate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | rate of synthesis is fold slower than rate of synthesis | Brevundimonas diminuta | 7-aminocephalosporanate + glutarate | - |
r | |
glutaryl-7-aminocephalosporanic acid + H2O | rate of synthesis is slower than rate of hydrolysis | Pseudomonas sp. | 7-aminocephalosporanate + glutarate | - |
r | |
glutaryl-7-aminocephalosporanic acid + H2O | rate of synthesis is slower than rate of hydrolysis | Brevibacillus laterosporus | 7-aminocephalosporanate + glutarate | - |
r | |
glutaryl-7-aminocephalosporanic acid + H2O | rate of synthesis is fold slower than rate of synthesis | Brevundimonas diminuta N176 | 7-aminocephalosporanate + glutarate | - |
r | |
glutaryl-7-aminocephalosporanic acid + H2O | rate of synthesis is slower than rate of hydrolysis | Brevibacillus laterosporus J1 | 7-aminocephalosporanate + glutarate | - |
r | |
N-acetyl cephalosporin C + H2O | activity is 1% of the activity with glutaryl-7-aminocephalosporanic acid | Brevundimonas diminuta | acetate + cephalosporin C | - |
? | |
N-acetyl cephalosporin C + H2O | activity is 1% of the activity with glutaryl-7-aminocephalosporanic acid | Brevundimonas diminuta N176 | acetate + cephalosporin C | - |
? | |
succinyl-7-aminocephalosporanic acid + H2O | activity is 32% of the activity with glutaryl-7-aminocephalosporanic acid | Brevundimonas diminuta | 7-aminocephalosporanate + succinate | - |
? | |
succinyl-7-aminocephalosporanic acid + H2O | activity is 32% of the activity with glutaryl-7-aminocephalosporanic acid | Brevundimonas diminuta N176 | 7-aminocephalosporanate + succinate | - |
? | |
succinyl-7-aminocephalosporanic acid + H2O | activity is 6.1% of the activity with glutaryl-7-aminocephalosporanic acid | Brevibacillus laterosporus | 7-aminocephalosporanic acid + succinate | - |
? | |
succinyl-7-aminocephalosporanic acid + H2O | activity is 9.9fold higher than with glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. | 7-aminocephalosporanic acid + succinate | - |
? | |
succinyl-7-aminocephalosporanic acid + H2O | activity is 6.1% of the activity with glutaryl-7-aminocephalosporanic acid | Brevibacillus laterosporus J1 | 7-aminocephalosporanic acid + succinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 1 * 70000, SDS-PAGE | Brevibacillus laterosporus |
? | 1 * 26000 + 1 * 58000, SDS-PAGE | Brevundimonas diminuta |
dimer | 1 * 28000 + 1 * 61000, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
cephalosporin C acylase | - |
Pseudomonas sp. |
cephalosporin C acylase | - |
Brevibacillus laterosporus |
cephalosporin C acylase | - |
Brevundimonas diminuta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Brevibacillus laterosporus |
40 | - |
- |
Brevundimonas diminuta |
48 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | 30°C: about 45% of maximal activity, 55°C: about 70% of maximal activity | Pseudomonas sp. |
30 | 55 | 30°C: about 45% of maximal activity, 55°C: about 70% of maximal activity | Brevibacillus laterosporus |
30 | 55 | 30°C: about 45% of maximal activity, 55°C: about 70% of maximal activity | Brevundimonas diminuta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
1 h, less than 10% loss of activity at pH 6.0, about 60% less of activity at pH 5, about 35% loss of activity at pH 7, about 50% loss of activity at pH 8, about 90% loss of activity at pH 9 | Pseudomonas sp. |
50 | - |
1 h, less than 10% loss of activity at pH 7.0, about 20% less of activity at pH 5, about 35% loss of activity at pH 7, about 70% loss of activity at pH 8, about 90% loss of activity at pH 9 | Brevibacillus laterosporus |
50 | - |
1 h, stable at pH 9.0, about 45% less of activity at pH 6, about 15% loss of activity at pH 7, about 10% loss of activity at pH 8, about 60% loss of activity at pH 10 | Brevundimonas diminuta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reaction with glutaryl-7-aminocephalosporanic acid | Brevibacillus laterosporus |
9 | - |
reaction with glutaryl-7-aminocephalosporanic acid | Brevundimonas diminuta |
9 | 10 | reaction with glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6: about 50% of maximal activity, pH 90: about 90% of maximal activity, hydrolysis of glutaryl-7-aminocephalosporanic acid | Brevibacillus laterosporus |
7 | 9 | activity decrease from pH 7 to pH 9, synthesis of glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. |
7 | 9 | activity decreases from pH 7 to pH 9, synthesis of glutaryl-7-aminocephalosporanic acid | Brevibacillus laterosporus |
8 | 10 | pH 8: about 50% of maximal activity, pH 9-10: maximal activity, hydrolysis of glutaryl-7-aminocephalosporanic acid | Pseudomonas sp. |
8 | - |
activity decrease from pH 7 to pH 9, synthesis of glutaryl-7-aminocephalosporanic acid | Brevundimonas diminuta |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
7-aminocephalosporanate | - |
Brevundimonas diminuta | |
2 | - |
7-aminocephalosporanate | - |
Brevibacillus laterosporus | |
2.3 | - |
7-aminocephalosporanate | - |
Pseudomonas sp. | |
2.5 | - |
Glutarate | - |
Brevundimonas diminuta | |
10 | - |
Glutarate | - |
Pseudomonas sp. | |
53 | - |
Glutarate | - |
Brevibacillus laterosporus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Brevundimonas diminuta | isoelectric focusing | - |
4.5 |
Brevibacillus laterosporus | isoelectric focusing | - |
4.7 |
Pseudomonas sp. | above, isoelectric focusing | - |
6.5 |