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Literature summary for 3.5.1.93 extracted from

  • Otten, L.G.; Sio, C.F.; van der Sloot, A.M.; Cool, R.H.; Quax, W.J.
    Mutational analysis of a key residue in the substrate specificity of a cephalosporin acylase (2004), Chembiochem, 5, 820-825.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
N266A turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 2.3fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.8fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2.9fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 8.4fold higher than wild-type value Pseudomonas sp. SY-77-1
N266C turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.3fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.5fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 5.2fold higher than wild-type value Pseudomonas sp. SY-77-1
N266D turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 205fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.8fold higher than wild-type value. Less efficient cleavage of the spacer from the alpha-subunit compared to wild-type enzyme Pseudomonas sp. SY-77-1
N266E turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 17.8fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 11.5fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 160fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 9.4fold higher than wild-type value. Less efficient cleavage of the spacer from the alpha-subunit compared to wild-type enzyme Pseudomonas sp. SY-77-1
N266F turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.1fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 2fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 4.4fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 3.2fold higher than wild-type value Pseudomonas sp. SY-77-1
N266G turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.8fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 17.4fold higher than wild-type value Pseudomonas sp. SY-77-1
N266H turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.15fold higher than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 8.6fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 1.3fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 1.4fold higher than wild-type value. 100% improved conversion of cephalosporin C compared to wild type enzyme Pseudomonas sp. SY-77-1
N266L turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.64fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 48.8fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 13.9fold higher than wild-type value Pseudomonas sp. SY-77-1
N266M turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 4.6fold higher than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 3.6fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2.2fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 5.4fold higher than wild-type value Pseudomonas sp. SY-77-1
N266P turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 6.7fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 3.7fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 30.8fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 41.9fold higher than wild-type value Pseudomonas sp. SY-77-1
N266Q turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.12fold higher than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.9fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 1.7fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 3.9fold higher wild-type value. 100% improved conversion of cephalosporin C compared to wild type enzyme Pseudomonas sp. SY-77-1
N266S turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.7fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2.5fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 2.4fold higher than wild-type value Pseudomonas sp. SY-77-1
N266T turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 2.7fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 4.75fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 8.2fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 48.4fold higher than wild-type value Pseudomonas sp. SY-77-1
N266W turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 2fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 3fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 5.8fold higher than wild-type value. 30% improved conversion of cephalosporin C compared to wild type enzyme Pseudomonas sp. SY-77-1
N266Y turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.8fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 6.6fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 3.5fold higher than wild-type value Pseudomonas sp. SY-77-1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
glutaryl-7-aminocephalosporanic acid wild-type enzyme Pseudomonas sp. SY-77-1
0.044
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266H Pseudomonas sp. SY-77-1
0.075
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266S Pseudomonas sp. SY-77-1
0.1
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266F Pseudomonas sp. SY-77-1
0.11
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266Y Pseudomonas sp. SY-77-1
0.12
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266Q Pseudomonas sp. SY-77-1
0.14
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266H Pseudomonas sp. SY-77-1
0.16
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266C Pseudomonas sp. SY-77-1
0.17
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266M Pseudomonas sp. SY-77-1
0.18
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266W Pseudomonas sp. SY-77-1
0.26
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266A Pseudomonas sp. SY-77-1
0.29
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266E Pseudomonas sp. SY-77-1
0.33
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266M Pseudomonas sp. SY-77-1
0.43
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266L Pseudomonas sp. SY-77-1
0.54
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266G Pseudomonas sp. SY-77-1
0.59
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266W Pseudomonas sp. SY-77-1
0.6
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266F Pseudomonas sp. SY-77-1
0.62
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266Q Pseudomonas sp. SY-77-1
0.65
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266Y Pseudomonas sp. SY-77-1
0.8
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266C Pseudomonas sp. SY-77-1
0.99
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266S Pseudomonas sp. SY-77-1
1
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266L Pseudomonas sp. SY-77-1
1.2
-
adipyl-7-aminodeacetoxycephalosporanic acid wild-type enzyme Pseudomonas sp. SY-77-1
1.3
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266P Pseudomonas sp. SY-77-1
1.4
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266G Pseudomonas sp. SY-77-1
1.5
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266T Pseudomonas sp. SY-77-1
2.2
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266A Pseudomonas sp. SY-77-1
2.2
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266D Pseudomonas sp. SY-77-1
4.4
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266P Pseudomonas sp. SY-77-1
5.7
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266T Pseudomonas sp. SY-77-1
13.8
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266E Pseudomonas sp. SY-77-1

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. SY-77-1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adipyl-7-aminodeacetoxycephalosporanic acid + H2O
-
Pseudomonas sp. SY-77-1 adipate + 7-aminodeacetoxycephalosporanic acid
-
?
cephalosporin C + H2O low activity Pseudomonas sp. SY-77-1 cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
?
glutaryl-7-aminocephalosporanic acid + H2O
-
Pseudomonas sp. SY-77-1 7-aminocephalosporanate + glutarate
-
?

Synonyms

Synonyms Comment Organism
cephalosporin acylase
-
Pseudomonas sp. SY-77-1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266D Pseudomonas sp. SY-77-1
0.023
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266E Pseudomonas sp. SY-77-1
0.025
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266E Pseudomonas sp. SY-77-1
0.061
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266P Pseudomonas sp. SY-77-1
0.082
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266L Pseudomonas sp. SY-77-1
0.13
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266P Pseudomonas sp. SY-77-1
0.15
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266T Pseudomonas sp. SY-77-1
0.18
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266A Pseudomonas sp. SY-77-1
0.23
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266G Pseudomonas sp. SY-77-1
0.24
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266S Pseudomonas sp. SY-77-1
0.25
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266L Pseudomonas sp. SY-77-1
0.31
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266C Pseudomonas sp. SY-77-1
0.33
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266W Pseudomonas sp. SY-77-1
0.33
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266Y Pseudomonas sp. SY-77-1
0.37
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266F Pseudomonas sp. SY-77-1
0.41
-
adipyl-7-aminodeacetoxycephalosporanic acid wild-type enzyme Pseudomonas sp. SY-77-1
0.46
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266Q Pseudomonas sp. SY-77-1
0.47
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266H Pseudomonas sp. SY-77-1
0.49
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266T Pseudomonas sp. SY-77-1
0.61
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266Y Pseudomonas sp. SY-77-1
0.9
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266F Pseudomonas sp. SY-77-1
1.3
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266W Pseudomonas sp. SY-77-1
1.4
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266A Pseudomonas sp. SY-77-1
1.6
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266S Pseudomonas sp. SY-77-1
1.8
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266M Pseudomonas sp. SY-77-1
1.9
-
adipyl-7-aminodeacetoxycephalosporanic acid mutant enzyme N266M Pseudomonas sp. SY-77-1
2
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266C Pseudomonas sp. SY-77-1
2
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266G Pseudomonas sp. SY-77-1
2.4
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266Q Pseudomonas sp. SY-77-1
3.1
-
glutaryl-7-aminocephalosporanic acid mutant enzyme N266H Pseudomonas sp. SY-77-1
4
-
glutaryl-7-aminocephalosporanic acid wild-type enzyme Pseudomonas sp. SY-77-1