Protein Variants | Comment | Organism |
---|---|---|
N266A | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 2.3fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.8fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2.9fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 8.4fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
N266C | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.3fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.5fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 5.2fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
N266D | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 205fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.8fold higher than wild-type value. Less efficient cleavage of the spacer from the alpha-subunit compared to wild-type enzyme | Pseudomonas sp. SY-77-1 |
N266E | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 17.8fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 11.5fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 160fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 9.4fold higher than wild-type value. Less efficient cleavage of the spacer from the alpha-subunit compared to wild-type enzyme | Pseudomonas sp. SY-77-1 |
N266F | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.1fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 2fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 4.4fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 3.2fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
N266G | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.8fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 17.4fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
N266H | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.15fold higher than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 8.6fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 1.3fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 1.4fold higher than wild-type value. 100% improved conversion of cephalosporin C compared to wild type enzyme | Pseudomonas sp. SY-77-1 |
N266L | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.64fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 48.8fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 13.9fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
N266M | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 4.6fold higher than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 3.6fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2.2fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 5.4fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
N266P | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 6.7fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 3.7fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 30.8fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 41.9fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
N266Q | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.12fold higher than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.9fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 1.7fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 3.9fold higher wild-type value. 100% improved conversion of cephalosporin C compared to wild type enzyme | Pseudomonas sp. SY-77-1 |
N266S | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.7fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2.5fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 2.4fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
N266T | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 2.7fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 4.75fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 8.2fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 48.4fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
N266W | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 2fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 3fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 5.8fold higher than wild-type value. 30% improved conversion of cephalosporin C compared to wild type enzyme | Pseudomonas sp. SY-77-1 |
N266Y | turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.8fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 6.6fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 3.5fold higher than wild-type value | Pseudomonas sp. SY-77-1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
glutaryl-7-aminocephalosporanic acid | wild-type enzyme | Pseudomonas sp. SY-77-1 | |
0.044 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266H | Pseudomonas sp. SY-77-1 | |
0.075 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266S | Pseudomonas sp. SY-77-1 | |
0.1 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266F | Pseudomonas sp. SY-77-1 | |
0.11 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266Y | Pseudomonas sp. SY-77-1 | |
0.12 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266Q | Pseudomonas sp. SY-77-1 | |
0.14 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266H | Pseudomonas sp. SY-77-1 | |
0.16 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266C | Pseudomonas sp. SY-77-1 | |
0.17 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266M | Pseudomonas sp. SY-77-1 | |
0.18 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266W | Pseudomonas sp. SY-77-1 | |
0.26 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266A | Pseudomonas sp. SY-77-1 | |
0.29 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266E | Pseudomonas sp. SY-77-1 | |
0.33 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266M | Pseudomonas sp. SY-77-1 | |
0.43 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266L | Pseudomonas sp. SY-77-1 | |
0.54 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266G | Pseudomonas sp. SY-77-1 | |
0.59 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266W | Pseudomonas sp. SY-77-1 | |
0.6 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266F | Pseudomonas sp. SY-77-1 | |
0.62 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266Q | Pseudomonas sp. SY-77-1 | |
0.65 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266Y | Pseudomonas sp. SY-77-1 | |
0.8 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266C | Pseudomonas sp. SY-77-1 | |
0.99 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266S | Pseudomonas sp. SY-77-1 | |
1 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266L | Pseudomonas sp. SY-77-1 | |
1.2 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | wild-type enzyme | Pseudomonas sp. SY-77-1 | |
1.3 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266P | Pseudomonas sp. SY-77-1 | |
1.4 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266G | Pseudomonas sp. SY-77-1 | |
1.5 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266T | Pseudomonas sp. SY-77-1 | |
2.2 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266A | Pseudomonas sp. SY-77-1 | |
2.2 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266D | Pseudomonas sp. SY-77-1 | |
4.4 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266P | Pseudomonas sp. SY-77-1 | |
5.7 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266T | Pseudomonas sp. SY-77-1 | |
13.8 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266E | Pseudomonas sp. SY-77-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. SY-77-1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adipyl-7-aminodeacetoxycephalosporanic acid + H2O | - |
Pseudomonas sp. SY-77-1 | adipate + 7-aminodeacetoxycephalosporanic acid | - |
? | |
cephalosporin C + H2O | low activity | Pseudomonas sp. SY-77-1 | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. SY-77-1 | 7-aminocephalosporanate + glutarate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cephalosporin acylase | - |
Pseudomonas sp. SY-77-1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266D | Pseudomonas sp. SY-77-1 | |
0.023 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266E | Pseudomonas sp. SY-77-1 | |
0.025 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266E | Pseudomonas sp. SY-77-1 | |
0.061 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266P | Pseudomonas sp. SY-77-1 | |
0.082 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266L | Pseudomonas sp. SY-77-1 | |
0.13 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266P | Pseudomonas sp. SY-77-1 | |
0.15 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266T | Pseudomonas sp. SY-77-1 | |
0.18 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266A | Pseudomonas sp. SY-77-1 | |
0.23 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266G | Pseudomonas sp. SY-77-1 | |
0.24 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266S | Pseudomonas sp. SY-77-1 | |
0.25 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266L | Pseudomonas sp. SY-77-1 | |
0.31 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266C | Pseudomonas sp. SY-77-1 | |
0.33 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266W | Pseudomonas sp. SY-77-1 | |
0.33 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266Y | Pseudomonas sp. SY-77-1 | |
0.37 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266F | Pseudomonas sp. SY-77-1 | |
0.41 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | wild-type enzyme | Pseudomonas sp. SY-77-1 | |
0.46 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266Q | Pseudomonas sp. SY-77-1 | |
0.47 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266H | Pseudomonas sp. SY-77-1 | |
0.49 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266T | Pseudomonas sp. SY-77-1 | |
0.61 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266Y | Pseudomonas sp. SY-77-1 | |
0.9 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266F | Pseudomonas sp. SY-77-1 | |
1.3 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266W | Pseudomonas sp. SY-77-1 | |
1.4 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266A | Pseudomonas sp. SY-77-1 | |
1.6 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266S | Pseudomonas sp. SY-77-1 | |
1.8 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266M | Pseudomonas sp. SY-77-1 | |
1.9 | - |
adipyl-7-aminodeacetoxycephalosporanic acid | mutant enzyme N266M | Pseudomonas sp. SY-77-1 | |
2 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266C | Pseudomonas sp. SY-77-1 | |
2 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266G | Pseudomonas sp. SY-77-1 | |
2.4 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266Q | Pseudomonas sp. SY-77-1 | |
3.1 | - |
glutaryl-7-aminocephalosporanic acid | mutant enzyme N266H | Pseudomonas sp. SY-77-1 | |
4 | - |
glutaryl-7-aminocephalosporanic acid | wild-type enzyme | Pseudomonas sp. SY-77-1 |