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Literature summary for 3.5.1.93 extracted from

  • Lee, Y.H.; Chang, T.S.; Liu, H.J.; Chu, W.S.
    An acidic glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas nitroreducens (1998), Biotechnol. Appl. Biochem., 28, 113-118.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
7-aminocephalosporanate 20 mM, 81% inhibition Pseudomonas nitroreducens
cephalosporin C 20 mM, 28% inhibition Pseudomonas nitroreducens
Glutaric acid 20 mM, 28% loss of activity Pseudomonas nitroreducens
succinyl-7-aminocephalosporanic acid 20 mM, 23% loss of activity Pseudomonas nitroreducens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.92
-
L-gamma-glutamyl p-nitroanilide
-
Pseudomonas nitroreducens
10.41
-
glutaryl-7-aminocephalosporanic acid
-
Pseudomonas nitroreducens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
1 * 21000 + 1 * 35000, SDS-PAGE Pseudomonas nitroreducens
35000
-
1 * 21000 + 1 * 35000, SDS-PAGE Pseudomonas nitroreducens
58000
-
gel filtration Pseudomonas nitroreducens

Organism

Organism UniProt Comment Textmining
Pseudomonas nitroreducens
-
CCRC 11041
-
Pseudomonas nitroreducens CCRC 11041
-
CCRC 11041
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas nitroreducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaryl-7-aminocephalosporanic acid + H2O
-
Pseudomonas nitroreducens 7-aminocephalosporanate + glutarate
-
?
glutaryl-7-aminocephalosporanic acid + H2O
-
Pseudomonas nitroreducens CCRC 11041 7-aminocephalosporanate + glutarate
-
?
L-gamma-glutamyl p-nitroanilide + H2O
-
Pseudomonas nitroreducens L-glutamate + p-nitroaniline
-
?
L-gamma-glutamyl p-nitroanilide + H2O
-
Pseudomonas nitroreducens CCRC 11041 L-glutamate + p-nitroaniline
-
?
additional information enzyme also exhibits L-gamma-glutamyltranspeptidase activity Pseudomonas nitroreducens ?
-
?
additional information enzyme also exhibits L-gamma-glutamyltranspeptidase activity Pseudomonas nitroreducens CCRC 11041 ?
-
?

Subunits

Subunits Comment Organism
dimer 1 * 21000 + 1 * 35000, SDS-PAGE Pseudomonas nitroreducens

Synonyms

Synonyms Comment Organism
GL-7-ACA acylase
-
Pseudomonas nitroreducens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
43
-
-
Pseudomonas nitroreducens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 55 15°C: about 40% of maximal activity, 30°C: about 55% of maximal activity, 45°C: about 75% of maximal activity, 55°C: about 30% of maximal activity Pseudomonas nitroreducens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 5.5, 30 min, stable up to Pseudomonas nitroreducens
55
-
pH 5.5, 30 min, about 55% loss of activity Pseudomonas nitroreducens
60
-
pH 5.5, 30 min, complete loss of activity Pseudomonas nitroreducens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Pseudomonas nitroreducens

pH Range

pH Minimum pH Maximum Comment Organism
5 6 pH 5.0: about 70% of maximal activity, pH 6.0: about 75% of maximal activity, pH 4.5: about 20% of maximal activity, pH 6.5: about 15% of maximal activity Pseudomonas nitroreducens

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5
-
37°C, 30 min, about 75% loss of activity Pseudomonas nitroreducens
4 6.5 37°C, 30 min, stable Pseudomonas nitroreducens
10
-
37°C, 30 min, about 39% loss of activity Pseudomonas nitroreducens

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas nitroreducens isoelectric focusing, pH-range 3.5-9.5
-
5.3