Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7-aminocephalosporanate | 20 mM, 81% inhibition | Pseudomonas nitroreducens | |
cephalosporin C | 20 mM, 28% inhibition | Pseudomonas nitroreducens | |
Glutaric acid | 20 mM, 28% loss of activity | Pseudomonas nitroreducens | |
succinyl-7-aminocephalosporanic acid | 20 mM, 23% loss of activity | Pseudomonas nitroreducens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.92 | - |
L-gamma-glutamyl p-nitroanilide | - |
Pseudomonas nitroreducens | |
10.41 | - |
glutaryl-7-aminocephalosporanic acid | - |
Pseudomonas nitroreducens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
1 * 21000 + 1 * 35000, SDS-PAGE | Pseudomonas nitroreducens |
35000 | - |
1 * 21000 + 1 * 35000, SDS-PAGE | Pseudomonas nitroreducens |
58000 | - |
gel filtration | Pseudomonas nitroreducens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas nitroreducens | - |
CCRC 11041 | - |
Pseudomonas nitroreducens CCRC 11041 | - |
CCRC 11041 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas nitroreducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas nitroreducens | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas nitroreducens CCRC 11041 | 7-aminocephalosporanate + glutarate | - |
? | |
L-gamma-glutamyl p-nitroanilide + H2O | - |
Pseudomonas nitroreducens | L-glutamate + p-nitroaniline | - |
? | |
L-gamma-glutamyl p-nitroanilide + H2O | - |
Pseudomonas nitroreducens CCRC 11041 | L-glutamate + p-nitroaniline | - |
? | |
additional information | enzyme also exhibits L-gamma-glutamyltranspeptidase activity | Pseudomonas nitroreducens | ? | - |
? | |
additional information | enzyme also exhibits L-gamma-glutamyltranspeptidase activity | Pseudomonas nitroreducens CCRC 11041 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 21000 + 1 * 35000, SDS-PAGE | Pseudomonas nitroreducens |
Synonyms | Comment | Organism |
---|---|---|
GL-7-ACA acylase | - |
Pseudomonas nitroreducens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
- |
Pseudomonas nitroreducens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 55 | 15°C: about 40% of maximal activity, 30°C: about 55% of maximal activity, 45°C: about 75% of maximal activity, 55°C: about 30% of maximal activity | Pseudomonas nitroreducens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 5.5, 30 min, stable up to | Pseudomonas nitroreducens |
55 | - |
pH 5.5, 30 min, about 55% loss of activity | Pseudomonas nitroreducens |
60 | - |
pH 5.5, 30 min, complete loss of activity | Pseudomonas nitroreducens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Pseudomonas nitroreducens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | pH 5.0: about 70% of maximal activity, pH 6.0: about 75% of maximal activity, pH 4.5: about 20% of maximal activity, pH 6.5: about 15% of maximal activity | Pseudomonas nitroreducens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
37°C, 30 min, about 75% loss of activity | Pseudomonas nitroreducens |
4 | 6.5 | 37°C, 30 min, stable | Pseudomonas nitroreducens |
10 | - |
37°C, 30 min, about 39% loss of activity | Pseudomonas nitroreducens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas nitroreducens | isoelectric focusing, pH-range 3.5-9.5 | - |
5.3 |