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Literature summary for 3.5.1.93 extracted from

  • Ishiye, M.; Niwa, M.
    Nucleotide sequence and expression in Escherichia coli of the cephalosporin acylase gene of a Pseudomonas strain (1992), Biochim. Biophys. Acta, 1132, 233-239.
    View publication on PubMed
Search for more literature for 3.5.1.93

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli in a two-cistron system Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8
-
 L-gamma-glutamyl-p-nitroanilide
-
 Pseudomonas sp.
6.1
-
 glutaryl-7-aminocephalosporanic acid
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 1 * 40000 + 1 * 22000, SDS-PAGE Pseudomonas sp.
40000
-
 1 * 40000 + 1 * 22000, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. V22
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.6
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaryl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. 7-aminocephalosporanate + glutarate
-
 ?
glutaryl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. V22 7-aminocephalosporanate + glutarate
-
 ?
L-gamma-glutamyl p-nitroanilide + H2O
-
 Pseudomonas sp. L-glutamate + p-nitroaniline
-
 ?
L-gamma-glutamyl p-nitroanilide + H2O
-
 Pseudomonas sp. V22 L-glutamate + p-nitroaniline
-
 ?
additional information purified enzyme exhibits also gamma-glutamyltranspeptidase activity with L-gamma-glutamyl-p-nitroanilide Pseudomonas sp. ?
-
 ?
additional information purified enzyme exhibits also gamma-glutamyltranspeptidase activity with L-gamma-glutamyl-p-nitroanilide Pseudomonas sp. V22 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 40000 + 1 * 22000, SDS-PAGE Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. isoelectric focusing
-
 4.6