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Literature summary for 3.5.1.93 extracted from

  • Park, S.W.; Choi, S.Y.; Chung, K.H.; H43ong, S.I.; Kim, S.W.
    Characteristics of GL-7-ACA acylase immobilized on silica gel through silanization (2002), Biochem. Eng. J., 11, 87-93.
No PubMed abstract available

Application

Application Comment Organism
pharmacology the product of the reaction 7-aminocephalosporanic acid is a starting material for semisynthetic cephalosporin antibiotics. High thermal stability of the enzyme immobilized on silica gels indicates that it can be successfully used for the production of 7-aminocephalosporanic acid on an industrial scale Pseudomonas sp.

General Stability

General Stability Organism
the stability of the enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane is significantly improved over its free form at lower pH values Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.9
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 37°C, soluble enzyme Pseudomonas sp.
15.4
-
glutaryl-7-aminocephalosporanic acid pH 8.0, 37°C, enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
KAC-1
-
Pseudomonas sp. KAC-1
-
KAC-1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaryl-7-aminocephalosporanic acid + H2O
-
Pseudomonas sp. 7-aminocephalosporanate + glutarate
-
?
glutaryl-7-aminocephalosporanic acid + H2O
-
Pseudomonas sp. KAC-1 7-aminocephalosporanate + glutarate
-
?

Synonyms

Synonyms Comment Organism
GL-7-ACA acylase
-
Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
soluble enzyme and enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: soluble enzyme shows 35% of maximal activity, enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane shows 45% of maximal activity, 60°C: soluble enzyme shows 20% of maximal activity, enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane shows 40% of maximal activity Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermal stability of the enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane is increased significantly compared to that of the soluble enzyme Pseudomonas sp.
37
-
1 h, soluble enzyme and enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane are stable, t1/2 is 307.7 min for the soluble enzyme and 733.7 min for the immobilized enzyme Pseudomonas sp.
45
-
t1/2 is 49.97 min for the soluble enzyme and 200 min for the immobilized enzyme Pseudomonas sp.
50
-
t1/2 is 29.72 min for the soluble enzyme and 65.9 min for the immobilized enzyme Pseudomonas sp.
55
-
1 h, soluble enzyme loses 77% of activity. t1/2 is 17.03 min for the soluble enzyme and 34.74 min for the immobilized enzyme Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
soluble enzyme Pseudomonas sp.
8.3
-
enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
pH-dependent activity profile for the enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane is considerably expanded Pseudomonas sp.
6 10 pH 6.0: soluble enzyme shows 35% of maximal activity, enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane shows 60% of maximal activity, pH 10.0: soluble enzyme shows 67% of maximal activity, enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane shows 75% of maximal activity Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
the stability of the enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane is significantly improved over its free form at lower pH values Pseudomonas sp.