General Stability | Organism |
---|---|
Eupergit C-immobilized acylase has a half-life of greater than 16 days | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
glutaryl deacetoxy 7-aminocephalosporanic acid | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
1 * 65000 + 1 * 24000 | Pseudomonas sp. |
65000 | - |
1 * 65000 + 1 * 24000 | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. BL072 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O | low activity | Pseudomonas sp. | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
cephalosporin C + H2O | low activity | Pseudomonas sp. BL072 | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
glutaryl deacetoxy 7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. | deacetoxycephalosporanic acid + glutarate | - |
? | |
glutaryl deacetoxy 7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. BL072 | deacetoxycephalosporanic acid + glutarate | - |
? | |
glutaryl deacetyl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. | deacetyl-7-aminocephalosporanic acid + glutarate | - |
? | |
glutaryl deacetyl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. BL072 | deacetyl-7-aminocephalosporanic acid + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. | 7-aminocephalosporanate + glutarate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. BL072 | 7-aminocephalosporanate + glutarate | - |
? | |
additional information | the enzyme has transacylase activity 10times that of its hydrolytic activity | Pseudomonas sp. | ? | - |
? | |
additional information | the enzyme has transacylase activity 10times that of its hydrolytic activity | Pseudomonas sp. BL072 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 65000 + 1 * 24000 | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
glutaryl-7-aminocephalosporanic acid acylase | - |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas sp. | - |
- |
8.5 |