Application | Comment | Organism |
---|---|---|
synthesis | immobilized mutant enzyme M269Y/C305S may be a promising enzyme in one-step enzymatic production of 7-aminocephalosporanate from cephalosporin C | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
C102S | mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C | Pseudomonas sp. |
C199S | mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C | Pseudomonas sp. |
C277S | mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C | Pseudomonas sp. |
C305S | mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C. Expression in Escherichia coli is 2-3fold higher than that of the wild-type enzyme | Pseudomonas sp. |
C391S | mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C | Pseudomonas sp. |
C493S | mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C | Pseudomonas sp. |
C496S | mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C | Pseudomonas sp. |
C748S | mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C | Pseudomonas sp. |
M269Y/C305S | 1.6fold higher activity with cephalosporin C than wild-type enzyme | Pseudomonas sp. |
General Stability | Organism |
---|---|
immobilized mutant enzyme M269Y/C305S is effective in more than 60 cycles for the production of 7-aminocephalosporanate | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PCMB | - |
Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
N176 | - |
Storage Stability | Organism |
---|---|
25°C, 4 weeks, mutant enzyme C305S loses 25% of its activity, mutant enzyme M269Y/C305S loses 25% of its activity | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O | - |
Pseudomonas sp. | cephalosporanic acid + 2-amino-5-hydroxypentanoate | - |
? | |
glutaryl-7-aminocephalosporanic acid + H2O | - |
Pseudomonas sp. | 7-aminocephalosporanate + glutarate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cephalosporin C acylase | - |
Pseudomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.102 | - |
PCMB | - |
Pseudomonas sp. |