Any feedback?
Literature summary for 3.5.1.92 extracted from
- A fluorescent assay suitable for inhibitor screening and vanin tissue quantification (2010), Anal. Biochem., 399, 284-292.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the highly sensitive fluorescent assay for the hydrolysis of pantothenate-7-amido-4-methylcoumarin as substrate to pantothenic acid and 7-amino-4-methylcoumarin could be a powerful tool for target validation and drug lead identification and characterization | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in CHO cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-bis[(2-hydroxyethyl)thio]-1,4-naphthoquinone | i.e. NSC 95397, from library of pharmacologically active compounds, LOPAC | Homo sapiens |  |
| 2-mercaptoethanol | reduced vanin-1 activity | Homo sapiens | |
| 5-(N,N-Hexamethylene)amiloride | from library of pharmacologically active compounds, LOPAC | Homo sapiens | |
| 8-cyclopentyl-1,3-dipropylxanthine | from library of pharmacologically active compounds, LOPAC | Homo sapiens | |
| beta-lapachone | from library of pharmacologically active compounds, LOPAC | Homo sapiens | |
| dithiothreitol | vanin-1 activity is reduced in the absence of dithiothreitol and in the presence of a high level of 50 mM dithiothreitol | Homo sapiens | |
| N2-(cis-2-aminocyclohexyl)-N6-(3-chlorophenyl)-9-ethyl-9H-purine-2,6-diamine hydrochloride | i.e. CGP-74514A hydrochloride, from library of pharmacologically active compounds, LOPAC | Homo sapiens | |
| roscovitine | from library of pharmacologically active compounds, LOPAC | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13 | - |
pantothenate-7-amido-4-methylcoumarin | apparent Km value, soluble recombinant vanin-1, pH 7.7, 25°C | Homo sapiens | |
| 28 | - |
pantothenate-7-amido-4-methylcoumarin | apparent Km value, HPLC assay, pH 7.7, 25°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the purified recombinant vanin-1 consists of a extracellular portion without a glycosylphosphatidylinositol linker | Homo sapiens | - |
- |
| soluble | an highly sensitive fluorescent assay using a novel fluorescent pantotheine derivative is used for characterization of a soluble version of human vanin-1 recombinant protein, identification of hits from high-throughput screening, and quantification of vanin pantotheinase acitivity in cell lines and tissues | Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | important for vanin-1 activity, vanin-1 activity is reduced in the absence of dithiothreitol and in the presence of a high level of 50 mM dithiothreitol. Optimal concentration: 0.4 mM | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
size exclusion chromatography-multiangular light scattering (SEC-MALS) | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
C57BI/6 mice | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme,His- and Flag-tagged vanin-1 is purified over a 10 ml HisTrap Fast Flow column (a nickel affinity chromatography), 95% purity | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | higher level in kidney than in liver, determined by RT-PCR | Mus musculus | - |
| liver | higher level in kidney than in liver, similar expression in lung, determined by RT-PCR | Mus musculus | - |
| lung | similar expression like in liver, determined by RT-PCR | Mus musculus | - |
| NCI-H292 cell | lung epithelial cells, transfected and selected for stable expression of human vanin-1 | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pantetheine + H2O | development of a highly sensitive fluorescent assay using a novel fluorescently labeled pantotheine derivative for high throughput screening for drug development and for quantification in various cell types and tissues | Homo sapiens | pantothenic acid + cysteamine | - |
? | |
| pantothenate-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | pantothenic acid + 7-amino-4-methylcoumarin | based on LC-MS analysis | ? | |
| pantothenate-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | pantothenic acid + 7-amino-4-methylcoumarin | based on LC-MS analysis, demonstrating that pantothenate-7-amido-4-methylcoumarin is hydrolyzed by vanin-1 at the amide-bond | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pantetheinase | - |
Mus musculus |
| pantetheinase | - |
Homo sapiens |
| vanin-1 | - |
Mus musculus |
| vanin-1 | belongs to the nitrilase superfamily, which contains the invariant catalytic triad residues: glutamate, lysine, and cysteine | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
greatly reduced activity observed at both acidic (pH 3.0) and basic (pH 10.0) conditions | Homo sapiens |
| 6.5 | 8 | - |
Homo sapiens |
| 7.5 | - |
assay at | Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00404 | - |
pH 7.5, 25°C | Homo sapiens | 5-(N,N-Hexamethylene)amiloride | |
| 0.0057 | - |
pH 7.5, 25°C | Homo sapiens | 2,3-bis[(2-hydroxyethyl)thio]-1,4-naphthoquinone | |
| 0.0079 | - |
pH 7.5, 25°C | Homo sapiens | N2-(cis-2-aminocyclohexyl)-N6-(3-chlorophenyl)-9-ethyl-9H-purine-2,6-diamine hydrochloride | |
| 0.0095 | - |
pH 7.5, 25°C | Homo sapiens | beta-lapachone | |
| 0.0135 | - |
pH 7.5, 25°C | Homo sapiens | 8-cyclopentyl-1,3-dipropylxanthine | |
| 0.0176 | - |
pH 7.5, 25°C | Homo sapiens | roscovitine | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
