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Literature summary for 3.5.1.92 extracted from
- Continuous spectrophotometric assay of pantetheinase activity (1984), Anal. Biochem., 142, 175-181.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000028 | - |
S-cysteamine-3-pyruvate | pH 7.6, 37°C | Equus caballus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Equus caballus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Equus caballus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
a simple, rapid, very sensitive, and specific continous spectrophotometric assay of pantetheinase activity | Equus caballus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-pantetheine-3-pyruvate + H2O | - |
Equus caballus | S-cysteamine-3-pyruvate + pantothenate | S-cysteamine-3-pyruvate cyclizes in a non-rate-limiting step to give 2H-1,4-thiazin-5,6-dihydro-3-carboxylic acid, a compound exhibiting a strong absorption at 296 nm | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pantetheinase | - |
Equus caballus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Equus caballus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.7 | pH 5.5: about 50% of maximal activity, pH 9.7: about 70% of maximal activity | Equus caballus |
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