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Literature summary for 3.5.1.90 extracted from

  • Woodson, J.D.; Escalante-Semerena, J.C.
    CbiZ, an amidohydrolase enzyme required for salvaging the coenzyme B12 precursor cobinamide in archaea (2004), Proc. Natl. Acad. Sci. USA, 101, 3591-3596.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.90

Cloned(Commentary)

Cloned (Comment) Organism
cbiZ gene, overexpression in Escherichia coli Methanosarcina mazei

Protein Variants

Protein Variants Comment Organism
additional information inactivation of the cbiZ gene blocks the ability of the organism to salvage dicyanocobinamide Halobacterium sp.

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by KCN Methanosarcina mazei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenosylcobinamide + H2O Halobacterium sp. CbiZ is required for salvaging the coenzyme B12 precursor cobinamide in archaea adenosylcobyric acid + (R)-1-aminopropan-2-ol
-
 ?
adenosylcobinamide + H2O Methanosarcina mazei CbiZ is required for salvaging the coenzyme B12 precursor cobinamide in archaea adenosylcobyric acid + (R)-1-aminopropan-2-ol adenosylcobyric acid is a bona fide intermediate of the de novo coenzyme B12 biosynthetic route ?
adenosylcobinamide + H2O Methanosarcina mazei Goe1 CbiZ is required for salvaging the coenzyme B12 precursor cobinamide in archaea adenosylcobyric acid + (R)-1-aminopropan-2-ol adenosylcobyric acid is a bona fide intermediate of the de novo coenzyme B12 biosynthetic route ?
adenosylcobinamide + H2O Halobacterium sp. NRC-1 CbiZ is required for salvaging the coenzyme B12 precursor cobinamide in archaea adenosylcobyric acid + (R)-1-aminopropan-2-ol
-
 ?

Organism

Organism UniProt Comment Textmining
Halobacterium sp. Q9HPK9
-
-
Halobacterium sp. NRC-1 Q9HPK9
-
-
Methanosarcina mazei Q8Q0G3 strain Göl
-
Methanosarcina mazei Goe1 Q8Q0G3 strain Göl
-

Purification (Commentary)

Purification (Comment) Organism
recombinant CbiZ Methanosarcina mazei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
 pH 7.5, 37°C, dicyanocobinamide as substrate Methanosarcina mazei
6.4
-
 pH 7.5, 37°C, adenosylcobinamide as substrate Methanosarcina mazei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosylcobinamide + H2O
-
 Methanosarcina mazei adenosylcobyric acid + (R)-1-aminopropan-2-ol
-
 ?
adenosylcobinamide + H2O
-
 Halobacterium sp. adenosylcobyric acid + (R)-1-aminopropan-2-ol
-
 ?
adenosylcobinamide + H2O CbiZ is required for salvaging the coenzyme B12 precursor cobinamide in archaea Halobacterium sp. adenosylcobyric acid + (R)-1-aminopropan-2-ol
-
 ?
adenosylcobinamide + H2O CbiZ is required for salvaging the coenzyme B12 precursor cobinamide in archaea Methanosarcina mazei adenosylcobyric acid + (R)-1-aminopropan-2-ol adenosylcobyric acid is a bona fide intermediate of the de novo coenzyme B12 biosynthetic route ?
adenosylcobinamide + H2O
-
 Methanosarcina mazei Goe1 adenosylcobyric acid + (R)-1-aminopropan-2-ol
-
 ?
adenosylcobinamide + H2O CbiZ is required for salvaging the coenzyme B12 precursor cobinamide in archaea Methanosarcina mazei Goe1 adenosylcobyric acid + (R)-1-aminopropan-2-ol adenosylcobyric acid is a bona fide intermediate of the de novo coenzyme B12 biosynthetic route ?
adenosylcobinamide + H2O
-
 Halobacterium sp. NRC-1 adenosylcobyric acid + (R)-1-aminopropan-2-ol
-
 ?
adenosylcobinamide + H2O CbiZ is required for salvaging the coenzyme B12 precursor cobinamide in archaea Halobacterium sp. NRC-1 adenosylcobyric acid + (R)-1-aminopropan-2-ol
-
 ?
dicyanocobinamide + H2O 3fold reduced activity compared with adenosylcobinamide Methanosarcina mazei ?
-
 ?
dicyanocobinamide + H2O 3fold reduced activity compared with adenosylcobinamide Methanosarcina mazei Goe1 ?
-
 ?

Synonyms

Synonyms Comment Organism
AdoCbi amidohydrolase
-
 Methanosarcina mazei
CbiZ
-
 Methanosarcina mazei
CbiZ
-
 Halobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methanosarcina mazei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Methanosarcina mazei