Cloned (Comment) | Organism |
---|---|
gene CG9542, cloning from larvae, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli | Drosophila melanogaster |
Crystallization (Comment) | Organism |
---|---|
enzyme in complex with inhibitor PMSF, X-ray diffraction structure determination and analysis, molecular replacement using structure PDB ID 2PBL | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diazinon | - |
Drosophila melanogaster | |
diazoxon | - |
Drosophila melanogaster | |
PMSF | binding structure analysis, overview | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
N-formyl-L-kynurenine | pH 7.4, 22°C, recombinant enzyme | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-L-kynurenine + H2O | Drosophila melanogaster | - |
formate + L-kynurenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9VMC9 | gene CG9542 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by affinity chromatography, ion exchange chromatography, and gel filtration | Drosophila melanogaster |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N-formyl-L-kynurenine + H2O = formate + L-kynurenine | via tetrahedral intermediate, enzyme structure and catalytic mechanism, overview | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-L-kynurenine + H2O | - |
Drosophila melanogaster | formate + L-kynurenine | - |
? | |
N-formyl-L-kynurenine + H2O | analysis of enzyme-substrate complex structure using the crystal structure of enzyme-inhibitor PMSF complex and molecular docking, overview | Drosophila melanogaster | formate + L-kynurenine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
formylkynurenine formamidase | - |
Drosophila melanogaster |
KFase | - |
Drosophila melanogaster |
kynurenine formamidase | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Drosophila melanogaster |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26.4 | - |
N-formyl-L-kynurenine | pH 7.4, 22°C, recombinant enzyme | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Drosophila melanogaster |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, overview | Drosophila melanogaster |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Drosophila melanogaster | spectral changes do not lead to an exact isosbestic point, which might be due to the formation of intermediates or an initial protein conformation change caused by addition of the substrate | - |
additional information |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the alpha/beta hydrolase fold family | Drosophila melanogaster |
metabolism | second enzyme in the kynurenine pathway of tryptophan metabolism | Drosophila melanogaster |
additional information | active site structure, enzyme ligand binding, and catalytic mechanism, molecular docking study, structure modeling, overview | Drosophila melanogaster |
physiological function | maintaining or regulating kynurenine metabolism through the molecular and biochemical regulation of the enzyme, overview | Drosophila melanogaster |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
82.5 | - |
N-formyl-L-kynurenine | pH 7.4, 22°C, recombinant enzyme | Drosophila melanogaster |