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Literature summary for 3.5.1.9 extracted from

  • Kurnasov, O.; Jablonski, L.; Polanuyer, B.; Dorrestein, P.; Begley, T.; Osterman, A.
    Aerobic tryptophan degradation pathway in bacteria: novel kynurenine formamidase (2003), FEMS Microbiol. Lett., 227, 219-227.
    View publication on PubMed
Search for more literature for 3.5.1.9

Application

Application Comment Organism
degradation aerobic degradation of L-tryptophan Pseudomonas aeruginosa
degradation aerobic degradation of L-tryptophan Bacillus cereus
degradation aerobic degradation of L-tryptophan Cupriavidus metallidurans

Cloned(Commentary)

Cloned (Comment) Organism
expression of putative kynBAU in Escherichia coli Cupriavidus metallidurans
kynB gene, expression in Escherichia coli as N-terminal His6 tag fusions Pseudomonas aeruginosa
kynB gene, expression in Escherichia coli as N-terminal His6 tag fusions Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
EDTA 0.1 mM, complete inactivation Cupriavidus metallidurans
additional information no inhibition by PMSF Cupriavidus metallidurans

Metals/Ions

Metals/Ions Comment Organism Structure
Co3+ 15-20fold activation Cupriavidus metallidurans
Mn2+ 15-20fold activation Cupriavidus metallidurans
additional information no activation by Mg2+, Cu2+ and Ni3+ Cupriavidus metallidurans

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
 strain ATCC 14579
-
Cupriavidus metallidurans
-
-
-
Cupriavidus metallidurans CH34
-
-
-
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa
-
 Bacillus cereus
-
 Cupriavidus metallidurans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5
-
 1 mM N-formyl-L-kynurenine, pH 7.4, 37°C Cupriavidus metallidurans
4.7
-
 1 mM N-formyl-L-kynurenine, pH 7.4, 37°C Pseudomonas aeruginosa
5.9
-
 1 mM N-formyl-L-kynurenine, pH 7.4, 37°C Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methylformanilide + H2O
-
 Cupriavidus metallidurans 2-methylaniline + formate
-
 ?
2-methylformanilide + H2O
-
 Cupriavidus metallidurans CH34 2-methylaniline + formate
-
 ?
N-formyl-4-(methylamino)-benzoic acid + H2O
-
 Cupriavidus metallidurans 4-methylaminobenzoic acid + formate
-
 ?
N-formyl-4-(methylamino)-benzoic acid + H2O
-
 Cupriavidus metallidurans CH34 4-methylaminobenzoic acid + formate
-
 ?
N-formyl-L-kynurenine + H2O
-
 Pseudomonas aeruginosa L-kynurenine + formate
-
 ?
N-formyl-L-kynurenine + H2O
-
 Bacillus cereus L-kynurenine + formate
-
 ?
N-formyl-L-kynurenine + H2O
-
 Cupriavidus metallidurans L-kynurenine + formate
-
 ?
N-formyl-L-kynurenine + H2O
-
 Cupriavidus metallidurans CH34 L-kynurenine + formate
-
 ?

Synonyms

Synonyms Comment Organism
KFA
-
 Pseudomonas aeruginosa
KFA
-
 Bacillus cereus
KFA
-
 Cupriavidus metallidurans