Any feedback?
Literature summary for 3.5.1.9 extracted from
- Kynurenine fromamidase from Neurospora (1954), J. Biol. Chem., 207, 657-663.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-acetylanthranilic acid | 15% inhibition at 6.7 mM | Neurospora crassa |  |
| N-formyl-o-aminotoluene | 45% inhibition at 6.7 mM | Neurospora crassa | |
| Na-bisulfite | complete inhibition at 4 mM | Neurospora crassa | |
| NaCN | 31% inhibition at 5 mM | Neurospora crassa | |
| NaHSO3 | complete inhibition at 4 mM | Neurospora crassa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
N-formyl-L-kynurenine | - |
Neurospora crassa | |
| 2.5 | - |
N-formyl-o-aminonitrobenzene | - |
Neurospora crassa | |
| 2.8 | - |
N-formyl-o-aminoacetophenone | - |
Neurospora crassa | |
| 3.3 | - |
N-formylanthranilic acid | - |
Neurospora crassa | |
| 7.7 | - |
N-formyl-m-aminobenzoic acid | - |
Neurospora crassa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-kynurenine + H2O | Neurospora crassa | - |
L-kynurenine + formate | - |
? | |
| N-formyl-m-aminobenzoic acid + H2O | Neurospora crassa | very low activity | formate + m-aminobenzoic acid | - |
? | |
| N-formyl-o-aminoacetophenone + H2O | Neurospora crassa | 22% of activity compared to N-formyl-L-kynurenine | formate + o-aminoacetophenone | - |
? | |
| N-formyl-o-aminonitrobenzene + H2O | Neurospora crassa | low activity | formate + o-nitroaniline | - |
? | |
| N-formylanthranilic acid + H2O | Neurospora crassa | 24% of activity compared to N-formyl-L-kynurenine | formate + anthranilic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Neurospora crassa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Neurospora crassa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-kynurenine + H2O | - |
Neurospora crassa | L-kynurenine + formate | - |
? | |
| N-formyl-m-aminobenzoic acid + H2O | very low activity | Neurospora crassa | formate + m-aminobenzoic acid | - |
? | |
| N-formyl-o-aminoacetophenone + H2O | 22% of activity compared to N-formyl-L-kynurenine | Neurospora crassa | formate + o-aminoacetophenone | - |
? | |
| N-formyl-o-aminonitrobenzene + H2O | low activity | Neurospora crassa | formate + o-nitroaniline | - |
? | |
| N-formylanthranilic acid + H2O | 24% of activity compared to N-formyl-L-kynurenine | Neurospora crassa | formate + anthranilic acid | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 56 | - |
30% inactivation after 2 min | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 7.8 | - |
Neurospora crassa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
