Literature summary for 3.5.1.9 extracted from

Serrano, A.E.; Nagayama, F.
Inhibition studies on liver arylformamidases of rainbow trout and cattle (1991), Comp. Biochem. Physiol. B, 99, 281-285.

Activating Compound

Activating Compound	Comment	Organism
arginine	30% activation at 20 mM	Bos taurus
ascorbate	57% activation at 100 mM	Oncorhynchus mykiss
L-tryptophan	activation from 5-20 mM	Bos taurus
lysine	30% activation at 20 mM	Bos taurus
methionine	20% activation at 20 mM	Bos taurus

General Stability	Organism
ionic strength affects enzyme activity	Bos taurus
ionic strength affects enzyme activity	Oncorhynchus mykiss

Inhibitors	Comment	Organism
3-hydroxy-DL-kynurenine	50% inhibition at 0.2 mM, Ki: 8 mM	Oncorhynchus mykiss
anthranilic acid	9% inhibition at 0.5-1 mM	Bos taurus
anthranilic acid	29% inhibition at 1-2 mM	Oncorhynchus mykiss
ascorbate	50% inhibition at 10 mM	Bos taurus
Ba2+	strong inhibition at 1 mM	Bos taurus
Co2+	moderate inhibition at 1 mM	Bos taurus
Co2+	moderate inhibition at 1 mM	Oncorhynchus mykiss
Cu2+	moderate inhibition at 1 mM	Oncorhynchus mykiss
Hg2+	moderate inhibition at 1 mM	Bos taurus
Hg2+	strong inhibition at 1 mM	Oncorhynchus mykiss
isoleucine	19% inhibition at 20 mM	Oncorhynchus mykiss
L-kynurenine	marked inhibition above 0.1 mM	Bos taurus
L-kynurenine	competitive inhibition, Ki: 0.26 mM	Oncorhynchus mykiss
L-tryptophan	non-competitive inhibition, Ki: 5.24 mM	Oncorhynchus mykiss
methionine	20% inhibition at 20 mM	Oncorhynchus mykiss
Mg2+	moderate inhibition at 1 mM	Bos taurus
Mn2+	strong inhibition at 1 mM	Bos taurus
Mn2+	strong inhibition at 1 mM	Oncorhynchus mykiss
Na-bisulfite	50% inhibition at 0.18 mM	Bos taurus
Na-bisulfite	50% inhibition at 0.32 mM	Oncorhynchus mykiss
NaHSO3	50% inhibition at 0.18 mM	Bos taurus
NaHSO3	50% inhibition at 0.32 mM	Oncorhynchus mykiss
phenylalanine	slight inhibition at 20 mM	Bos taurus
phenylalanine	slight inhibition at 20 mM	Oncorhynchus mykiss
threonine	19% inhibition at 20 mM	Oncorhynchus mykiss
valine	slight inhibition at 20 mM	Bos taurus
Zn2+	moderate inhibition at 1 mM	Bos taurus
Zn2+	strong inhibition at 1 mM	Oncorhynchus mykiss

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-formyl-L-kynurenine + H2O	Bos taurus	-	L-kynurenine + formate	-	?
N-formyl-L-kynurenine + H2O	Oncorhynchus mykiss	-	L-kynurenine + formate	-	?

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-
Oncorhynchus mykiss	-	rainbow trout	-

Source Tissue	Comment	Organism	Textmining
liver	-	Bos taurus	-
liver	-	Oncorhynchus mykiss	-

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-formyl-L-kynurenine + H2O	-	Bos taurus	L-kynurenine + formate	-	?
N-formyl-L-kynurenine + H2O	-	Oncorhynchus mykiss	L-kynurenine + formate	-	?

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.18	-	NaHSO3	-	Bos taurus
0.18	-	Na-bisulfite	-	Bos taurus
0.32	-	NaHSO3	-	Oncorhynchus mykiss
5.24	-	L-tryptophan	non-competitive inhibition	Oncorhynchus mykiss
8	-	3-hydroxy-DL-kynurenine	-	Oncorhynchus mykiss
10	-	ascorbate	-	Bos taurus