Cloned (Comment) | Organism |
---|---|
gene GPI12, cloning in Escherichia coli strain Top 10 and tranformation and recombinant expression in Leishmaia major null mutant cells | Leishmania major |
Protein Variants | Comment | Organism |
---|---|---|
additional information | two alleles of the GPI12 gene in Leishmania major are successfully removed enabling the generation of a null mutant, which supports the idea that GPI12 is not an essential gene for the growth and survival of Leishmania and the homozygous knockouts of Leishmania are able to survive. Generation of a mutant parasite that causes no damaged to the host | Leishmania major |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | Q8I8A4 | isolated from infected BALB/c mice | - |
Leishmania major 75 | Q8I8A4 | isolated from infected BALB/c mice | - |
Leishmania major ER | Q8I8A4 | isolated from infected BALB/c mice | - |
Leishmania major IR | Q8I8A4 | isolated from infected BALB/c mice | - |
Leishmania major MRHO | Q8I8A4 | isolated from infected BALB/c mice | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | - |
Leishmania major | - |
promastigote | - |
Leishmania major | - |
Synonyms | Comment | Organism |
---|---|---|
GlcNAc-PI-de-N-acetylase | - |
Leishmania major |
GPI12 | - |
Leishmania major |
General Information | Comment | Organism |
---|---|---|
malfunction | an in vitro study using murine MQ cell line J774 shows that by the use of recombinant parasites the entry rate and infecting ability is decreased. The mutant parasites are unable to produce skin lesions even six months post-infection in the BALB/c mice | Leishmania major |
physiological function | GPI12 is not an essential gene for the growth and survival of Leishmania and the homozygous knockouts of Leishmania are able to survive. Stationary phase promastigotes are used for in vitro cell line J774 macrophage infectivity test also for in vivo infection | Leishmania major |