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Literature summary for 3.5.1.89 extracted from

  • Abdelwahab, N.Z.; Crossman, A.T.; Sullivan, L.; Ferguson, M.A.; Urbaniak, M.D.
    Inhibitors incorporating zinc-binding groups target the GlcNAc-PI de-N-acetylase in Trypanosoma brucei, the causative agent of African sleeping sickness (2012), Chem. Biol. Drug Des., 79, 270-278.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.89

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
1,5-anhydro-2-(carboxymethyl)-2-deoxy-D-glucitol
-
 Trypanosoma brucei
1,5-anhydro-2-deoxy-2-[2-(hydroxyamino)-2-oxoethyl]-D-glucitol
-
 Trypanosoma brucei
1,5-anhydro-3-O-benzyl-2-(carboxymethyl)-2-deoxy-D-glucitol
-
 Trypanosoma brucei
1,5-anhydro-3-O-benzyl-2-deoxy-2-[2-(hydroxyamino)-2-oxoethyl]-D-glucitol
-
 Trypanosoma brucei
2-(carboxymethyl)-2-deoxy-D-glucopyranose
-
 Trypanosoma brucei
phenyl 2-(carbamoylamino)-2-deoxy-1-thio-beta-D-glucopyranoside
-
 Trypanosoma brucei
phenyl 2-(carboxymethyl)-2-deoxy-1-thio-alpha-D-glucopyranoside
-
 Trypanosoma brucei
triethylammonium 1R,2R-1-O-[2-C-(carboxymethyl N-hydroxyamide)-2-deoxy-beta-D-glucopyranosyl]-cyclohexanediol 2-(n-octadecylphosphate)
-
 Trypanosoma brucei

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ a zinc metalloenzyme Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
 variant MITat1.4
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-GlcNAc-alpha(1->6)-D-myo-inositol-1-octadecyl phosphate + H2O
-
 Trypanosoma brucei D-GlcN-alpha(1->6)-D-myo-inositol-1-octadecyl phosphate + acetate
-
 ?
N-acetyl-D-glucosaminylphosphatidylinositol + H2O + H2O
-
 Trypanosoma brucei D-glucosaminylphosphatidylinositol + acetate
-
 ?

Synonyms

Synonyms Comment Organism
deNAc
-
 Trypanosoma brucei
GlcNAc-PI de-N-acetylase
-
 Trypanosoma brucei
N-acetylglucosamine-phosphatidylinositolde-N-acetylase
-
 Trypanosoma brucei

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.019
-
 in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei triethylammonium 1R,2R-1-O-[2-C-(carboxymethyl N-hydroxyamide)-2-deoxy-beta-D-glucopyranosyl]-cyclohexanediol 2-(n-octadecylphosphate)
0.1
-
 in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei phenyl 2-(carboxymethyl)-2-deoxy-1-thio-alpha-D-glucopyranoside
0.29
-
 in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei 1,5-anhydro-3-O-benzyl-2-(carboxymethyl)-2-deoxy-D-glucitol
0.3
-
 in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei 2-(carboxymethyl)-2-deoxy-D-glucopyranose
1.5
-
 in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei 1,5-anhydro-3-O-benzyl-2-deoxy-2-[2-(hydroxyamino)-2-oxoethyl]-D-glucitol
10
-
 IC50 above 10 mM, in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei phenyl 2-(carboxymethyl)-2-deoxy-1-thio-alpha-D-glucopyranoside
10
-
 IC50 above 10 mM, in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei 1,5-anhydro-2-(carboxymethyl)-2-deoxy-D-glucitol
10
-
 IC50 above 10 mM, in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei 1,5-anhydro-2-deoxy-2-[2-(hydroxyamino)-2-oxoethyl]-D-glucitol
10
-
 IC50 above 10 mM, in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30°C Trypanosoma brucei phenyl 2-(carbamoylamino)-2-deoxy-1-thio-beta-D-glucopyranoside