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Literature summary for 3.5.1.89 extracted from

  • Urbaniak, M.D.; Crossman, A.; Chang, T.; Smith, T.K.; van Aalten, D.M.; Ferguson, M.A.
    The N-acetyl-D-glucosaminylphosphatidylinositol De-N-acetylase of glycosylphosphatidylinositol biosynthesis is a zinc metalloenzyme (2005), J. Biol. Chem., 280, 22831-22838.
    View publication on PubMed
Search for more literature for 3.5.1.89

Activating Compound

Activating Compound Comment Organism Structure
additional information a tightly bound divalent metal cation is essential for activity Trypanosoma brucei
additional information a tightly bound divalent metal cation is essential for activity Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Trypanosoma brucei 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can replace Zn2+ Trypanosoma brucei
Co2+ can replace Zn2+ Rattus norvegicus
Cu2+ can replace Zn2+ Trypanosoma brucei
Cu2+ can replace Zn2+ Rattus norvegicus
Mg2+ can replace Zn2+ Trypanosoma brucei
Mg2+ can replace Zn2+ Rattus norvegicus
Ni2+ can replace Zn2+ Trypanosoma brucei
Ni2+ can replace Zn2+ Rattus norvegicus
Zn2+ essential for activity Trypanosoma brucei
Zn2+ essential for activity Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus O35790
-
-
Trypanosoma brucei Q382X3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-Acetyl-D-glucosaminylphosphatidylinositol + H2O 30°C, pH 7.4, 50 mM KCl, 10 mM dithiothreitol, 0.1 mM 1-chloro-3-tosylamido-7-amino-2-heptanone, 1 microg/ml leupeptin Trypanosoma brucei D-Glucosaminylphosphatidylinositol + acetate
-
 ?
N-Acetyl-D-glucosaminylphosphatidylinositol + H2O 37°C Rattus norvegicus D-Glucosaminylphosphatidylinositol + acetate
-
 ?

Synonyms

Synonyms Comment Organism
GlcNAc-PI de-N-acetylase
-
 Trypanosoma brucei
GlcNAc-PI de-N-acetylase
-
 Rattus norvegicus
N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase
-
 Trypanosoma brucei
N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase
-
 Rattus norvegicus