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Literature summary for 3.5.1.88 extracted from

  • Rodrigues-Poveda, C.A.; Perez-Moreno, G.; Vidal, A.E.; Urbina, J.A.; Gonzalez-Pacanowska, D.; Ruiz-Perez, L.M.
    Kinetic analyses and inhibition studies reveal novel features in peptide deformylase 1 from Trypanosoma cruzi (2012), Mol. Biochem. Parasitol., 182, 83-87.
    View publication on PubMed
Search for more literature for 3.5.1.88

Activating Compound

Activating Compound Comment Organism Structure
1,10-phenanthroline 1-5 mM strongly activates deformylase activity, activity increases 6fold at 1 mM Trypanosoma cruzi
EDTA 1-20 mM strongly activates deformylase activity, activity increases up to 70% at 10 mM Trypanosoma cruzi
N-formyl-Met-Ala-Ser the activity increases with substrate concentration up to 35 mM Trypanosoma cruzi

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by actinonin Trypanosoma cruzi
N-formyl-Met-Ala-Ser the enzyme activity of truncated PDF-1DeltaN37 is inhibited by an excess of N-formyl-Met-Ala-Ser substrate Trypanosoma cruzi
Ni2+ less than 40% residual activity at 0.1 mM Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
22.37
-
 N-formyl-Met-Ala-Ser truncated PDF-1DeltaN37, in 50 mM KH2PO4, pH 7.5, at 37°C Trypanosoma cruzi

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the enzyme is strongly activated by Cu2+ with 50% increase of activity at 0.05 mM Trypanosoma cruzi
additional information Co2+, Mg2+, Mn2+ do not significantly affect the enzyme activity Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
metal-affinity chromatography Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-formyl-Met-Ala-Ser + H2O
-
 Trypanosoma cruzi formate + Met-Ala-Ser
-
 ?

Subunits

Subunits Comment Organism
? x * 28000-30000, SDS-PAGE Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
PDF-1
-
 Trypanosoma cruzi
peptide deformylase 1
-
 Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.093
-
 N-formyl-Met-Ala-Ser truncated PDF-1DeltaN37, in 50 mM KH2PO4, pH 7.5, at 37°C Trypanosoma cruzi