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Literature summary for 3.5.1.88 extracted from
- Crystal structure of an EfPDF complex with Met-Ala-Ser based on crystallographic packing (2009), Biochem. Biophys. Res. Commun., 381, 630-633.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | peptide deformylase is a target protein for the development of anti-bacterial drugs | Enterococcus faecium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pET28a for expression in Escherichia coli BL21DE3 cells | Enterococcus faecium |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the crystal structure of peptide deformylase in complex with Met-Ala-Ser is determined to 2.5 A | Enterococcus faecium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecium | Q842S4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by affinity chromatography and gel filtration, after Ni-affinity chromatography the His tag is removed by thrombin cleavage and the protein is further purified on a HiLoad 26-60 Superdex-200 prep-grade column | Enterococcus faecium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EfPDF | - |
Enterococcus faecium |
| peptide deformylase | - |
Enterococcus faecium |
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Release 2023.1 (January 2023)
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