Application | Comment | Organism |
---|---|---|
drug development | potential target or the development of new antibacterial agents | Escherichia coli |
drug development | potential target or the development of new antibacterial agents | Geobacillus stearothermophilus |
drug development | potential target or the development of new antibacterial agents | Pseudomonas aeruginosa |
drug development | potential target or the development of new antibacterial agents | Staphylococcus aureus |
Cloned (Comment) | Organism |
---|---|
expressed in an Escherichia coli deficiency strain | Pseudomonas aeruginosa |
expressed in an Escherichia coli deficiency strain | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method | Escherichia coli |
vapor diffusion method | Geobacillus stearothermophilus |
vapor diffusion method | Pseudomonas aeruginosa |
vapor diffusion method | Staphylococcus aureus |
General Stability | Organism |
---|---|
Ni2+ and Co2+ increase stability compared to the Fe2+ enzyme | Geobacillus stearothermophilus |
Ni2+ and Co2+ increase stability compared to the Fe2+ enzyme | Pseudomonas aeruginosa |
Ni2+ and Co2+ increase stability compared to the Fe2+ enzyme | Staphylococcus aureus |
Ni2+ and Co2+ increases stability compared to the Fe2+ enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
actinonin | - |
Escherichia coli | |
actinonin | - |
Geobacillus stearothermophilus | |
actinonin | - |
Pseudomonas aeruginosa | |
actinonin | - |
Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can replace Fe2+ without loss of activity, enhances stability | Escherichia coli | |
Co2+ | can replace Fe2+ without loss of activity, enhances stability | Geobacillus stearothermophilus | |
Co2+ | can replace Fe2+ without loss of activity, enhances stability | Pseudomonas aeruginosa | |
Co2+ | can replace Fe2+ without loss of activity, enhances stability | Staphylococcus aureus | |
Fe2+ | required | Escherichia coli | |
Fe2+ | required | Geobacillus stearothermophilus | |
Fe2+ | required | Pseudomonas aeruginosa | |
Fe2+ | required | Staphylococcus aureus | |
Ni2+ | can replace Fe2+ without loss of activity, enhances stability | Escherichia coli | |
Ni2+ | can replace Fe2+ without loss of activity, enhances stability | Geobacillus stearothermophilus | |
Ni2+ | can replace Fe2+ without loss of activity, enhances stability | Pseudomonas aeruginosa | |
Ni2+ | can replace Fe2+ without loss of activity, enhances stability | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-L-methionine-polypeptide + H2O | Escherichia coli | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Geobacillus stearothermophilus | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Pseudomonas aeruginosa | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Staphylococcus aureus | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | Staphylococcus aureus RN4220 | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | formate + L-methionine-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6K3 | - |
- |
Geobacillus stearothermophilus | O31410 | - |
- |
Pseudomonas aeruginosa | Q9I7A8 | strain ATCC27853 | - |
Staphylococcus aureus | P68826 | - |
- |
Staphylococcus aureus RN4220 | P68826 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Geobacillus stearothermophilus |
recombinant enzyme | Pseudomonas aeruginosa |
recombinant enzyme | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-L-methionine-polypeptide + H2O | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | Escherichia coli | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | Geobacillus stearothermophilus | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | Pseudomonas aeruginosa | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | Staphylococcus aureus | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides | Staphylococcus aureus RN4220 | formate + L-methionine-polypeptide | - |
? |