Protein Variants | Comment | Organism |
---|---|---|
C102A | high level expression but no enzyme activity | Leptospira interrogans |
C102S | high level expression but no enzyme activity | Leptospira interrogans |
additional information | the variant with insertion residues R70Y71P72G73T74P75D76V77 displays no marked changes on enzymatic features | Leptospira interrogans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM | Leptospira interrogans | |
Cu2+ | 1 mM | Leptospira interrogans | |
Fe2+ | 1 mM | Leptospira interrogans | |
Mg2+ | 1 mM | Leptospira interrogans | |
Ni2+ | 1 mM | Leptospira interrogans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
formyl-Met-Ala-Ser | - |
Leptospira interrogans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | ratio of results of zinc to protein is 1:1 | Leptospira interrogans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20230 | - |
2 * 20230, mass spectrometry | Leptospira interrogans |
43400 | - |
gel filtration | Leptospira interrogans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leptospira interrogans | Q93LE9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Leptospira interrogans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
780.6 | - |
- |
Leptospira interrogans |
Storage Stability | Organism |
---|---|
4°C, stable for days | Leptospira interrogans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formyl-Met-Ala-Ser + H2O | - |
Leptospira interrogans | formate + Met-Ala-Ser | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 20230, mass spectrometry | Leptospira interrogans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 65 | - |
Leptospira interrogans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | 50°C: about 75% of maximal activity, 80°C: about 70% of maximal activity | Leptospira interrogans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-life: 17.15 min | Leptospira interrogans |
55 | - |
half-life: 1.82 min | Leptospira interrogans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
formyl-Met-Ala-Ser | - |
Leptospira interrogans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10.5 | - |
Leptospira interrogans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | pH 7.0: about 70% of maximal activity, pH 11.0: about 65% of maximal activity | Leptospira interrogans |