Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.88 extracted from

  • Serero, A.; Giglione, C.; Meinnel, T.
    Distinctive features of the two classes of eukaryotic peptide deformylases (2001), J. Mol. Biol., 314, 695-708.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
actinonin
-
Arabidopsis thaliana
actinonin
-
Escherichia coli
imino[(5-methoxy-5-oxo-4-[2-(sulfanylmethyl)hexanoyl]aminopentyl)amino]methaneamine
-
Arabidopsis thaliana
imino[(5-methoxy-5-oxo-4-[2-(sulfanylmethyl)hexanoyl]aminopentyl)amino]methaneamine
-
Escherichia coli
methyl-1-(sulfanylmethyl)hexanoate
-
Arabidopsis thaliana
methyl-1-(sulfanylmethyl)hexanoate
-
Escherichia coli
Phe-Arg-beta-naphthylamide
-
Arabidopsis thaliana
thiorphan
-
Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Arabidopsis thaliana
0.23
-
N-Formyl-Met-Leu-Phe enzyme form PDF1A Arabidopsis thaliana
0.25
-
N-formyl-Met-Ala-Ser enzyme form PDF1A Arabidopsis thaliana
0.27
-
N-formyl-Met-Ala-Ser enzyme form PDF1A Solanum lycopersicum
0.42
-
N-formyl-Arg(NH2) enzyme form PDF1A Arabidopsis thaliana
0.42
-
N-formyl-Ser(NH2) enzyme form PDF1A Arabidopsis thaliana
0.5
-
N-formyl-L-Arg-amide enzyme form Ni-PDF1B Arabidopsis thaliana
0.6
-
N-formyl-Nle-Ala-Ser enzyme form PDF1A Arabidopsis thaliana
0.74
-
N-formyl-Asp(NH2) enzyme form PDF1A Arabidopsis thaliana
0.79
-
N-formyl-Leu(NH2) enzyme form PDF1A Arabidopsis thaliana
1
-
N-formyl-Asp(NH2) enzyme form Ni-PDF1B Arabidopsis thaliana
1
-
N-formyl-Ser(NH2) enzyme form Ni-PDF1B Arabidopsis thaliana
2
-
N-formyl-Met-Ser-Asn-Glu enzyme form Ni-PDF1B Arabidopsis thaliana
2.9
-
N-formyl-Gly(NH2) enzyme form PDF1A Arabidopsis thaliana
3
-
N-formyl-Phe-Ala-Ser enzyme form PDF1A Arabidopsis thaliana
4.1
-
N-formyl-Met-Ser-Asn-Glu enzyme form PDF1A Arabidopsis thaliana
4.3
-
N-formyl-Gly(NH2) enzyme form Ni-PDF1B Arabidopsis thaliana
5.6
-
N-formyl-Met-Ala-Ser enzyme form Ni-PDF1B Arabidopsis thaliana
8
-
N-formyl-Met-Leu-Glu enzyme form PDF1A Arabidopsis thaliana
8.2
-
N-formyl-Met-Ala-Ser enzyme form PDF1B Arabidopsis thaliana
9.4
-
N-formyl-Nva-Ala-Ser enzyme form PDF1A Arabidopsis thaliana
11
-
N-formyl-Nle-Ala-Ser enzyme form Ni-PDF1B Arabidopsis thaliana
12
-
N-formyl-Met-Leu-Glu enzyme form Ni-PDF1B Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe
-
Escherichia coli
Fe PDF1B is an iron enzyme Arabidopsis thaliana
Zn PDF1A is a zinc enzyme, contains 0.8 mol of Zn per mol of enzyme Arabidopsis thaliana
Zn PDF1A is a zinc enzyme, contains 0.8 mol of Zn per mol of enzyme Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-formyl-L-methionine-polypeptide + H2O Escherichia coli
-
formate + L-methionine-polypeptide
-
?
N-formyl-L-methionine-polypeptide + H2O Arabidopsis thaliana deformylase is likely to play an essential role in the chloroplast formate + L-methionine-polypeptide
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
two classes of PDF: PDF1A and PDF1B
-
Escherichia coli
-
-
-
Solanum lycopersicum
-
two classes of PDF: PDF1A and PDF1B
-

Purification (Commentary)

Purification (Comment) Organism
two classes of PDF: PDF1A and PDF1B Arabidopsis thaliana
two classes of PDF: PDF1A and PDF1B Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Arabidopsis thaliana
-

Storage Stability

Storage Stability Organism
-20°C, PDF1A is stable for at least 6 months Arabidopsis thaliana
-20°C, PDF1A is stable for at least 6 months Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-formyl-Asp(NH2) + H2O
-
Arabidopsis thaliana ?
-
?
N-formyl-Gly(NH2) + H2O
-
Arabidopsis thaliana ?
-
?
N-formyl-L-Arg(NH2) + H2O
-
Arabidopsis thaliana ?
-
?
N-formyl-L-Arg-amide + H2O
-
Arabidopsis thaliana ?
-
?
N-formyl-L-methionine-polypeptide + H2O
-
Escherichia coli formate + L-methionine-polypeptide
-
?
N-formyl-L-methionine-polypeptide + H2O deformylase is likely to play an essential role in the chloroplast Arabidopsis thaliana formate + L-methionine-polypeptide
-
?
N-formyl-Leu(NH2) + H2O
-
Arabidopsis thaliana ?
-
?
N-formyl-Met-Ala + H2O
-
Escherichia coli formate + Met-Ala
-
?
N-formyl-Met-Ala + H2O
-
Arabidopsis thaliana formate + Met-Ala
-
?
N-formyl-Met-Ala(OCH3) + H2O
-
Escherichia coli formate + Met-Ala(OCH3)
-
?
N-formyl-Met-Ala(OCH3) + H2O
-
Arabidopsis thaliana formate + Met-Ala(OCH3)
-
?
N-formyl-Met-Ala-Ser + H2O
-
Escherichia coli formate + Met-Ala-Ser
-
?
N-formyl-Met-Ala-Ser + H2O
-
Arabidopsis thaliana formate + Met-Ala-Ser
-
?
N-formyl-Met-Ala-Ser + H2O
-
Solanum lycopersicum formate + Met-Ala-Ser
-
?
N-formyl-Met-Leu-Glu + H2O
-
Escherichia coli formate + Met-Leu-Glu
-
?
N-formyl-Met-Leu-Glu + H2O
-
Arabidopsis thaliana formate + Met-Leu-Glu
-
?
N-formyl-Met-Leu-p-nitroanilide + H2O
-
Escherichia coli formate + Met-Leu-p-nitroanilide
-
?
N-formyl-Met-Leu-p-nitroanilide + H2O
-
Arabidopsis thaliana formate + Met-Leu-p-nitroanilide
-
?
N-formyl-Met-Leu-Phe + H2O
-
Escherichia coli formate + Met-Leu-Phe
-
?
N-formyl-Met-Leu-Phe + H2O
-
Arabidopsis thaliana formate + Met-Leu-Phe
-
?
N-formyl-Met-Ser-Asn-Glu + H2O
-
Escherichia coli formate + Met-Ser-Asn-Glu
-
?
N-formyl-Met-Ser-Asn-Glu + H2O
-
Arabidopsis thaliana formate + Met-Ser-Asn-Glu
-
?
N-formyl-Nle-Ala(NH2) + H2O
-
Escherichia coli formate + Nle-Ala(NH2)
-
?
N-formyl-Nle-Ala(NH2) + H2O
-
Arabidopsis thaliana formate + Nle-Ala(NH2)
-
?
N-formyl-Nle-Ala-Ser + H2O
-
Escherichia coli formate + Nle-Ala-Ser
-
?
N-formyl-Nle-Ala-Ser + H2O
-
Arabidopsis thaliana formate + Nle-Ala-Ser
-
?
N-formyl-Nle-Arg(NH2) + H2O
-
Escherichia coli formate + Nle-Arg(NH2)
-
?
N-formyl-Nle-Arg(NH2) + H2O
-
Arabidopsis thaliana formate + Nle-Arg(NH2)
-
?
N-formyl-Nle-Asp(NH2) + H2O
-
Escherichia coli formate + Nle-Asp(NH2)
-
?
N-formyl-Nle-Asp(NH2) + H2O
-
Arabidopsis thaliana formate + Nle-Asp(NH2)
-
?
N-formyl-Nle-Gly(NH2) + H2O
-
Escherichia coli formate + Nle-Gly(NH2)
-
?
N-formyl-Nle-Gly(NH2) + H2O
-
Arabidopsis thaliana formate + Nle-Gly(NH2)
-
?
N-formyl-Nle-Leu(NH2) + H2O
-
Escherichia coli formate + Nle-Leu(NH2)
-
?
N-formyl-Nle-Leu(NH2) + H2O
-
Arabidopsis thaliana formate + Nle-Leu(NH2)
-
?
N-formyl-Nle-NH(CH3) + H2O
-
Escherichia coli formate + Nle-NH(CH3)
-
?
N-formyl-Nle-NH(CH3) + H2O
-
Arabidopsis thaliana formate + Nle-NH(CH3)
-
?
N-formyl-Nle-NH2 + H2O
-
Escherichia coli formate + Nle-Nle-NH2
-
?
N-formyl-Nle-NH2 + H2O
-
Arabidopsis thaliana formate + Nle-Nle-NH2
-
?
N-formyl-Nle-Ser(NH2) + H2O
-
Escherichia coli formate + Nle-Ser(NH2)
-
?
N-formyl-Nle-Ser(NH2) + H2O
-
Arabidopsis thaliana formate + Nle-Ser(NH2)
-
?
N-formyl-Nva-Ala-Ser + H2O
-
Escherichia coli formate + Nva-Ala-Ser
-
?
N-formyl-Nva-Ala-Ser + H2O
-
Arabidopsis thaliana formate + Nva-Ala-Ser
-
?
N-formyl-Phe-Ala-Ser + H2O
-
Escherichia coli formate + Phe-Ala-Ser
-
?
N-formyl-Phe-Ala-Ser + H2O
-
Arabidopsis thaliana formate + Phe-Ala-Ser
-
?
N-formyl-Ser(NH2) + H2O
-
Arabidopsis thaliana ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Arabidopsis thaliana
0.36
-
N-formyl-Nle-Leu(NH2) enzyme form PDF1A Arabidopsis thaliana
0.4
-
N-formyl-Nle-Asp(NH2) enzyme form PDF1A Arabidopsis thaliana
0.6
-
N-formyl-Phe-Ala-Ser enzyme form PDF1A Arabidopsis thaliana
0.7
-
N-Formyl-Met-Leu-Phe enzyme form PDF1A Arabidopsis thaliana
1.1
-
N-formyl-Met-Leu-Glu enzyme form PDF1A Arabidopsis thaliana
1.4
-
N-formyl-Nle-Gly(NH2) enzyme form PDF1A Arabidopsis thaliana
1.9
-
N-formyl-Met-Ser-Asn-Glu enzyme form Ni-PDF1B Arabidopsis thaliana
5
-
N-formyl-Nle-Arg(NH2) enzyme form PDF1A Arabidopsis thaliana
6.4
-
N-formyl-Nle-Asp(NH2) enzyme form Ni-PDF1B Arabidopsis thaliana
9.2
-
N-formyl-Met-Leu-Glu enzyme form Ni-PDF1B Arabidopsis thaliana
10
-
N-formyl-Nle-Ser(NH2) enzyme form PDF1A Arabidopsis thaliana
11
-
N-formyl-Met-Ser-Asn-Glu enzyme form PDF1A Arabidopsis thaliana
13
-
N-formyl-Met-Ala-Ser enzyme form Ni-PDF1B Arabidopsis thaliana
15
-
N-formyl-Met-Ala-Ser enzyme form PDF1A Solanum lycopersicum
18
-
N-formyl-Nva-Ala-Ser enzyme form PDF1A Arabidopsis thaliana
22
-
N-formyl-Met-Ala-Ser enzyme form PDF1A Arabidopsis thaliana
26
-
N-formyl-Nle-Ala-Ser enzyme form PDF1A Arabidopsis thaliana
51
-
N-formyl-Nle-Ser(NH2) enzyme form Ni-PDF1B Arabidopsis thaliana
75
-
N-formyl-Met-Ala-Ser enzyme form Ni-PDF1B Arabidopsis thaliana
136
-
N-formyl-Nle-Ala-Ser enzyme form Ni-PDF1B Arabidopsis thaliana
138
-
N-formyl-Nle-Arg(NH2) enzyme form Ni-PDF1B Arabidopsis thaliana