Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
General Stability | Organism |
---|---|
extremely labile | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | competitive | Escherichia coli | |
1,2-Ethanedithiol | irreversible | Escherichia coli | |
1,3-propanedithiol | irreversible | Escherichia coli | |
1,5-pentanedithiol | - |
Escherichia coli | |
2,3-dimercapto-1-propanesulfonic acid | - |
Escherichia coli | |
2,3-dimercapto-1-propanol | - |
Escherichia coli | |
2-mercaptoethanol | 30% inhibition at 1 mM | Escherichia coli | |
EDTA | slight inhibition at 10 mM | Escherichia coli | |
glutathione | 10% inhibition at 1 mM | Escherichia coli | |
p-chloromercuribenzoate | potent inhibitor, IC50: 0.07 mM | Escherichia coli | |
Thiophenol | IC50: 0.5 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
N-formylmethionyl-Leu-p-nitroanilide | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19200 | - |
gel filtration, electrospray ionization mass spectrometry | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formyl-L-methionine-polypeptide + H2O | Escherichia coli | requires at least a dipeptide as substrate, increasing activity with increasing peptide length until it reaches the tetrapeptide | formate + L-methionine-polypeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, recombinant enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
-70°C, 33% glycerol, 2 years | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formyl-L-Met-Leu-p-nitroanilide + H2O | - |
Escherichia coli | formate + Met-Leu-p-nitroanilide | - |
? | |
N-formyl-Ala-Gly-Ser-Glu + H2O | poor activity, preference of N-terminal methionine | Escherichia coli | formate + Ala-Gly-Ser-Glu | - |
? | |
N-formyl-His-Ala-Ser-Arg + H2O | greatly attenuated activity, preference for N-terminal methionine | Escherichia coli | formate + His-Ala-Ser-Arg | - |
? | |
N-formyl-L-Met-D-Ala-Ser-Arg + H2O | strong requirement for L-methionine, but D-amino acids tolerated in other positions | Escherichia coli | formate + L-Met-D-Ala-Ser-Arg | - |
? | |
N-formyl-L-methionine-polypeptide + H2O | requires at least a dipeptide as substrate, increasing activity with increasing peptide length until it reaches the tetrapeptide | Escherichia coli | formate + L-methionine-polypeptide | - |
? | |
N-formyl-L-Nle-L-Ala-L-Ser + H2O | norleucine is the only excepted substitution for methionine | Escherichia coli | formate + L-Nle-L-Ala-L-Ser | - |
? | |
N-formyl-Met + H2O | poor activity | Escherichia coli | formate + Met | - |
? | |
N-formyl-Met-Leu-p-nitroanilide + H2O | best substrate | Escherichia coli | formate + Met-Leu-p-nitroanilide | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 19200, electrospray ionization mass spectrometry | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
rapidly inactivated within 18-22°C in 2 min | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
sharp decrease of activity below and above | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
1,3-propanedithiol | - |
Escherichia coli | |
0.004 | - |
1,2-Ethanedithiol | - |
Escherichia coli | |
0.0109 | - |
2,3-dimercapto-1-propanol | - |
Escherichia coli | |
0.038 | - |
1,5-pentanedithiol | - |
Escherichia coli | |
0.0402 | - |
2,3-dimercapto-1-propanesulfonic acid | - |
Escherichia coli | |
0.17 | - |
1,10-phenanthroline | - |
Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.07 | - |
potent inhibitor, IC50: 0.07 mM | Escherichia coli | p-chloromercuribenzoate | |
0.5 | - |
IC50: 0.5 mM | Escherichia coli | Thiophenol |