Any feedback?
Literature summary for 3.5.1.88 extracted from
- Control of peptide deformylase activity by metal cations (1998), J. Mol. Biol., 280, 515-523.
General Stability
| General Stability | Organism |
|---|---|
| additon of metal ions required during purification | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Escherichia coli |  |
| Zn2+ | converting enzyme to inactive state | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
N-formyl-methionine | nickel-containing enzyme | Escherichia coli | |
| 3.5 | - |
N-formyl-Nle-Ala-Ser | nickel-containing enzyme | Escherichia coli | |
| 3.9 | - |
N-formyl-Met-Ala-Ser | nickel-containing enzyme | Escherichia coli | |
| 4 | - |
N-formyl-L-Met-L-Lys | nickel-containing enzyme | Escherichia coli | |
| 5.5 | - |
N-formyl-Met-Ser-Asn-Glu | nickel-containing enzyme | Escherichia coli | |
| 6.2 | - |
N-formyl-Met-Ala | nickel-containing enzyme | Escherichia coli | |
| 52 | - |
N-formyl-L-Ala-L-Ala-L-Ala | nickel-containing enzyme | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 250fold increase of activity | Escherichia coli | |
| Ni2+ | 450fold increasing activity, stabilizes active state | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
| Thermus thermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, Ni2+- and Mn2+-bound preparation | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | increasing activity by addition of Ni2+ or Mn2+ ions to the culture medium | Escherichia coli | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 97.2 | - |
Ni2+-bound enzyme | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-formyl-L-Ala-L-Ala-L-Ala + H2O | - |
Escherichia coli | ? | - |
? | |
| N-formyl-L-Met-L-Lys + H2O | - |
Escherichia coli | formate + L-Met-L-Lys | - |
? | |
| N-formyl-Met + H2O | minimal substrate | Escherichia coli | formate + Met | - |
? | |
| N-formyl-Met-Ala-Ser + H2O | - |
Thermus thermophilus | formate + Met-Ala-Ser | - |
? | |
| N-formyl-Met-Ala-Ser + H2O | - |
Escherichia coli | formate + Met-Ala-Ser | - |
? | |
| N-formyl-Met-Ser-Asn-Glu + H2O | - |
Escherichia coli | formate + Met-Ser-Asn-Glu | - |
? | |
| N-formyl-Nle-Ala-Ser + H2O | - |
Escherichia coli | formate + Nle-Ala-Ser | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
N-formyl-methionine | nickel-containing enzyme | Escherichia coli | |
| 5.7 | - |
N-formyl-L-Ala-L-Ala-L-Ala | nickel-containing enzyme | Escherichia coli | |
| 175 | - |
N-formyl-Met-Ser-Asn-Glu | nickel-containing enzyme | Escherichia coli | |
| 197 | - |
N-formyl-L-Met-L-Lys | nickel-containing enzyme | Escherichia coli | |
| 210 | - |
N-formyl-Met-Ala-Ser | nickel-containing enzyme | Escherichia coli | |
| 218 | - |
N-formyl-Nle-Ala-Ser | nickel-containing enzyme | Escherichia coli | |
| 232 | - |
N-formyl-Met-Ala | nickel-containing enzyme | Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
