Application | Comment | Organism |
---|---|---|
synthesis | development of a bienzymatic biocatalyst system comprising an N-succinylamino acid racemase from Geobacillus kaustophilus CECT4264 and the enantiospecific L-N-carbamoylase from Geobacillus stearothermophilus CECT43. The biocatalyst system is able to produce optically pure natural and non-natural L-amino acids starting from racemic mixtures of N-acetyl-, N-formyl- and N-carbamoyl-amino acids by dynamic kinetic resolution. The fastest conversion rate is found with N-formyl-aminoacids, followed by N-carbamoyl- and N-acetyl-amino acids, and the an N-succinylamino acid racemase proves to be the limiting step of the system due to its lower specific activity, overview | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
gene Bslcar, recombinant overexpression in Escherichia coli strain BL21 using the pJAVI80Rha plasmid | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | essential for activity | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-carbamoyl-DL-methionine + H2O | Geobacillus stearothermophilus | - |
L-methionine + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-methionine + H2O | Geobacillus stearothermophilus CECT43 | - |
L-methionine + CO2 + NH3 | - |
? | |
N-formyl-DL-methionine + H2O | Geobacillus stearothermophilus | - |
L-methionine + CO2 | - |
? | |
N-formyl-DL-methionine + H2O | Geobacillus stearothermophilus CECT43 | - |
L-methionine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q53389 | gene Bslcar | - |
Geobacillus stearothermophilus CECT43 | Q53389 | gene Bslcar | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is a strict enantiospecific L-N-carbamoylase. Development of a bienzymatic system comprising an N-succinylamino acid racemase from Geobacillus kaustophilus CECT4264 and the enantiospecific L-N-carbamoylase from Geobacillus stearothermophilus CECT43. The biocatalyst system is able to produce optically pure natural and non-natural L-amino acids starting from racemic mixtures of N-acetyl-, N-formyl- and N-carbamoyl-amino acids by dynamic kinetic resolution. The fastest conversion rate is found with N-formyl-aminoacids, followed by N-carbamoyl- and N-acetyl-amino acids, and the an N-succinylamino acid racemase proves to be the limiting stepof the system due to its lower specific activity, overview | Geobacillus stearothermophilus | ? | - |
? | |
additional information | the enzyme is a strict enantiospecific L-N-carbamoylase. Development of a bienzymatic system comprising an N-succinylamino acid racemase from Geobacillus kaustophilus CECT4264 and the enantiospecific L-N-carbamoylase from Geobacillus stearothermophilus CECT43. The biocatalyst system is able to produce optically pure natural and non-natural L-amino acids starting from racemic mixtures of N-acetyl-, N-formyl- and N-carbamoyl-amino acids by dynamic kinetic resolution. The fastest conversion rate is found with N-formyl-aminoacids, followed by N-carbamoyl- and N-acetyl-amino acids, and the an N-succinylamino acid racemase proves to be the limiting stepof the system due to its lower specific activity, overview | Geobacillus stearothermophilus CECT43 | ? | - |
? | |
N-carbamoyl-DL-2-aminoburyric acid + H2O | - |
Geobacillus stearothermophilus | L-2-aminobutyric acid + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-2-aminoburyric acid + H2O | - |
Geobacillus stearothermophilus CECT43 | L-2-aminobutyric acid + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-homophenylalanine + H2O | - |
Geobacillus stearothermophilus | L-homophenylalanine + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-homophenylalanine + H2O | - |
Geobacillus stearothermophilus CECT43 | L-homophenylalanine + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-methionine + H2O | - |
Geobacillus stearothermophilus | L-methionine + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-methionine + H2O | - |
Geobacillus stearothermophilus CECT43 | L-methionine + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-norleucine + H2O | - |
Geobacillus stearothermophilus | L-norleucine + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-norvaline + H2O | - |
Geobacillus stearothermophilus | L-norvaline + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-phenylglycine + H2O | low activity | Geobacillus stearothermophilus | L-phenylglycine + CO2 + NH3 | - |
? | |
N-formyl-DL-2-aminobutyric acid + H2O | - |
Geobacillus stearothermophilus | L-2-aminobutyric acid + CO2 | - |
? | |
N-formyl-DL-ethionine + H2O | - |
Geobacillus stearothermophilus | L-ethionine + CO2 | - |
? | |
N-formyl-DL-homophenylalanine + H2O | - |
Geobacillus stearothermophilus | L-homophenylalanine + CO2 | - |
? | |
N-formyl-DL-methionine + H2O | - |
Geobacillus stearothermophilus | L-methionine + CO2 | - |
? | |
N-formyl-DL-methionine + H2O | - |
Geobacillus stearothermophilus CECT43 | L-methionine + CO2 | - |
? | |
N-formyl-DL-norleucine + H2O | - |
Geobacillus stearothermophilus | L-norleucine + CO2 | - |
? | |
N-formyl-DL-norvaline + H2O | - |
Geobacillus stearothermophilus | L-norvaline + CO2 | - |
? | |
N-formyl-DL-phenylglycine + H2O | - |
Geobacillus stearothermophilus | L-phenylglycine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BsLcar | - |
Geobacillus stearothermophilus |
L-N-carbamoylase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 70 | biocatalyst system, optimum temperature ranges are 45-55°C for N-formyl-amino acids and 55-70°C for N-carbamoyl-derivatives | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
pH 8.0, the bienzymatic system retains almost total operability after 24 h of incubation at 45°C, overview | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
biocatalyst system for both N-formyl- and N-carbamoyl-amino acid substrates | Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
45°C, the bienzymatic system retains almost total operability after 24 h of incubation at pH 8.0, overview | Geobacillus stearothermophilus |