Application | Comment | Organism |
---|---|---|
additional information | enzyme immobilization on solid matrix results in a great enhancement of the enzyme activity toward N-formyl-tryptophan, the reaction can be repeated for several cycles, method optimization, overview | Geobacillus stearothermophilus |
synthesis | production of different optically pure L-alpha-amino acids starting from different racemic N-formyl- and N-carbamoyl-amino acids using a dynamic kinetic resolution approach with immobilized L-N-carbamoylase and N-succinyl-amino acid racemase as biocatalysts, the system is effective for the biosynthesis of natural and unnatural L-amino acids (enantiomeric excess over 99.5%), overview | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
gene Bslcar, recombinant expression | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme immobilization on solid matrix results in a great enhancement of the enzyme activity toward N-formyl-tryptophan, the reaction can be repeated for several cycles, method optimization, overview | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q53389 | gene Bslcar | - |
Geobacillus stearothermophilus CECT43 | Q53389 | gene Bslcar | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme by cobalt affinity chromatography, ultrafiltration and dialysis | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | catalytic promiscuity of L-N-carbamoylase from Geobacillus stearothermophilus CECT43, substrate specificity with different N-formyl- and N-carbamoyl-DL-amino acids, overview. No N-formyl-DL-tert-leucine | Geobacillus stearothermophilus | ? | - |
? | |
additional information | catalytic promiscuity of L-N-carbamoylase from Geobacillus stearothermophilus CECT43, substrate specificity with different N-formyl- and N-carbamoyl-DL-amino acids, overview. No N-formyl-DL-tert-leucine | Geobacillus stearothermophilus CECT43 | ? | - |
? | |
N-carbamoyl-DL-aminobutyric acid + H2O | - |
Geobacillus stearothermophilus | L-aminobutyric acid + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-homophenylalanine + H2O | - |
Geobacillus stearothermophilus | L-homophenylalanine + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-methionine + H2O | - |
Geobacillus stearothermophilus | L-methionine + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-methionine + H2O | - |
Geobacillus stearothermophilus CECT43 | L-methionine + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-norleucine + H2O | - |
Geobacillus stearothermophilus | L-norleucine + CO2 + NH3 | - |
? | |
N-carbamoyl-DL-norvaline + H2O | - |
Geobacillus stearothermophilus | L-norvaline + CO2 + NH3 | - |
? | |
N-formyl-DL-alanine + H2O | - |
Geobacillus stearothermophilus | L-alanine + CO2 | - |
? | |
N-formyl-DL-alanine + H2O | - |
Geobacillus stearothermophilus CECT43 | L-alanine + CO2 | - |
? | |
N-formyl-DL-aminobutyric acid + H2O | - |
Geobacillus stearothermophilus | L-aminobutyric acid + CO2 | - |
? | |
N-formyl-DL-ethionine + H2O | - |
Geobacillus stearothermophilus | L-ethionine + CO2 | - |
? | |
N-formyl-DL-homophenylalanine + H2O | - |
Geobacillus stearothermophilus | L-homophenylalanine + CO2 | - |
? | |
N-formyl-DL-methionine + H2O | - |
Geobacillus stearothermophilus | L-methionine + CO2 | - |
? | |
N-formyl-DL-methionine + H2O | - |
Geobacillus stearothermophilus CECT43 | L-methionine + CO2 | - |
? | |
N-formyl-DL-norleucine + H2O | - |
Geobacillus stearothermophilus | L-norleucine + CO2 | - |
? | |
N-formyl-DL-norvaline + H2O | - |
Geobacillus stearothermophilus | L-norvaline + CO2 | - |
? | |
N-formyl-DL-phenylalanine + H2O | - |
Geobacillus stearothermophilus | L-phenylalanine + CO2 | - |
? | |
N-formyl-DL-phenylglycine + H2O | - |
Geobacillus stearothermophilus | L-phenylglycine + CO2 | - |
? | |
N-formyl-DL-tryptophan + H2O | - |
Geobacillus stearothermophilus | L-tryptophan + CO2 | - |
? | |
N-formyl-DL-tryptophan + H2O | - |
Geobacillus stearothermophilus CECT43 | L-tryptophan + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BsLcar | - |
Geobacillus stearothermophilus |
L-N-carbamoylase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
assay at | Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | activity range | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | immobilized enzyme, in 100 mM borate-HCl, pH 8.0, 18 h, conversion rates decrease at temperatures over 55°C and are lost completely at over 70°C, for the immobilized enzyme system in production of optically pure L-amino acids, overview | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Geobacillus stearothermophilus |