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Literature summary for 3.5.1.86 extracted from

  • Kooo, C.W.
    Mandelamide hydrolase from Pseudomonas putida (1997), Diss. Abstr. Int. B, 58, 1865.
No PubMed abstract available
Search for more literature for 3.5.1.86

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
15
-
 (R)-(-)-mandelamide
-
 Pseudomonas putida
46
-
 (R)-(-)-mandelamide
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53810 53830 electrospray ionization mass spectrometry Pseudomonas putida
53850
-
 matrix-assisted laser desorption ionization Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 ATCC 12633
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-(-)-mandelamide + H2O
-
 Pseudomonas putida (R)-(-)mandelate + NH3
-
 ?
(S)-(+)-mandelamide + H2O
-
 Pseudomonas putida (S)-(+)-mandelate + NH3
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4
-
 (S)-(+)-mandelamide
-
 Pseudomonas putida
10.2
-
 (R)-(-)-mandelamide
-
 Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8
-
 Pseudomonas putida